Document Detail

Exercise training down-regulates hepatic lipogenic enzymes in meal-fed rats: fructose versus complex-carbohydrate diets.
MedLine Citation:
PMID:  9566986     Owner:  NLM     Status:  MEDLINE    
The maximal activity and mRNA abundance of hepatic fatty acid synthase (FAS) and other lipogenic enzymes were investigated in rats meal-fed either a high fructose (F) or a high cornstarch (C) diet. The diet contained 50% F or C (g/100 g), casein (20%), cornstarch (16.13%), corn oil (5%), minerals (5.37%), vitamins (1%) and Solka-floc (2%). Female Sprague-Dawley rats (n = 44) were randomly divided into C or F groups that were meal-fed for 3 h/d; each group was subdivided into exercise-trained (T) and untrained (U) groups. Treadmill training was performed 4 h after the initiation of the meal at 25 m/min, 10% grade for 2 h/d, 5 d/wk, for 10 wk. Rats were killed 9 h after the meal and 27 h after the last training session. F-fed rats had significantly higher activities of all lipogenic enzymes assayed and mRNA abundance of FAS and acetyl-coenzyme A carboxylase (ACC) than C rats (P < 0.05). Concentrations of plasma insulin and glucose and liver pyruvate were not altered by F feeding. Proportions of the fatty acids 18:2 and 20:4 were lower, whereas those of 16:0 and 16:1 were higher, in livers of F than of C rats (P < 0.05). Training decreased FAS activity by 50% (P < 0.05), without affecting FAS mRNA level in C rats; this down-regulation was absent in the F rats. ACC mRNA abundance tended to be lower in CT than in CU rats (P < 0.075). L-Type pyruvate kinase activity was lower in FT than in FU rats (P < 0.05), whereas other lipogenic enzyme activities did not differ between T and U rats of each diet group. We conclude that hepatic lipogenic enzyme induction by high carbohydrate meal feeding may be inhibited by exercise training and that a fructose-rich diet may attenuate this training-induced down-regulation.
R Fiebig; M A Griffiths; M T Gore; D H Baker; L Oscai; D M Ney; L L Ji
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  128     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  810-7     Citation Subset:  IM; S    
Departments of Kinesiology and Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA.
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MeSH Terms
Acetyl-CoA Carboxylase / genetics,  metabolism*
Blood Glucose / analysis
Body Weight / physiology
Citrate (si)-Synthase / metabolism
Cohort Studies
Dietary Carbohydrates / administration & dosage*
Fatty Acid Synthetase Complex / genetics,  metabolism*
Fatty Acids / analysis,  classification
Fructose / administration & dosage
Liver / chemistry,  enzymology*,  physiology
Muscle, Skeletal / enzymology
Organ Size / physiology
Physical Conditioning, Animal / physiology*
Pyruvate Kinase / metabolism
Pyruvic Acid / analysis
RNA, Messenger / analysis,  genetics
Random Allocation
Rats, Sprague-Dawley
Starch / administration & dosage
Grant Support
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Carbohydrates; 0/Fatty Acids; 0/RNA, Messenger; 127-17-3/Pyruvic Acid; 30237-26-4/Fructose; 9005-25-8/Starch; EC (si)-Synthase; EC Kinase; EC 6.-/Fatty Acid Synthetase Complex; EC Carboxylase

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