| Exercise training decreases hepatic SCD-1 gene expression and protein content in rats. | |
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MedLine Citation:
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PMID: 19777326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stearoyl-CoA desaturase-1 (SCD-1) is the rate limiting enzyme in the biosynthesis of saturated-derived monounsaturated fats that are the major constituents of very-low-density-lipoproteins-triacylglycerol (VLDL-TAG) and are involved in regulating cellular metabolism. The purpose of this study was to evaluate the effects of an 8-week exercise training program on the hepatic gene expression of this crucial enzyme. Female rats either trained (TR) or kept sedentary (Sed) for 8 weeks were submitted either to standard (SD) diet for 8 or for 6 weeks followed by high-fat (HF; 42% kcal) diet for 2 weeks. The 2-week-high fat feeding resulted in an increase in liver triacylgycerol (TAG), plasma free-fatty-acids (FFA), abdominal fat mass, sterol-regulatory-element-binding protein-1c (SREBP-1c), and carbohydrate-response-element-binding protein (ChREBP) gene expression in liver along with a decrease in SCD-1 gene expression and plasma and liver SCD-1 desaturation index (C16:1/C16:0). Liver TAG, plasma FFA, SREBP-1c mRNA, and SCD-1 desaturation indexes (C16:1/C16:0; C18:1/C18:0) were not changed in liver or in plasma by the training program. Nevertheless, training resulted in an important decrease in fat mass (P < 0.01), hepatic SCD-1 mRNA levels (P < 0.01), and protein content (P < 0.05) in both SD and HF fed rats. It is concluded that despite an absence of decreased liver TAG, exercise training contributes to the proper regulation of fat metabolism by down-regulating hepatic SCD-1 gene expression and protein content. |
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Authors:
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Siham Yasari; Denis Prud'homme; Donghao Wang; Marek Jankowski; Emile Levy; Jolanta Gutkowska; Jean-Marc Lavoie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-24 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 335 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-05-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 291-9 Citation Subset: IM |
Affiliation:
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Department of Kinesiology, University of Montreal, Montreal, Canada. syasari@uottawa.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics, metabolism Dietary Fats / metabolism Fatty Acids, Nonesterified / metabolism Female Gene Expression Lipid Metabolism Liver / enzymology*, metabolism Physical Conditioning, Animal* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Stearoyl-CoA Desaturase / genetics*, metabolism Sterol Regulatory Element Binding Protein 1 / genetics, metabolism Triglycerides / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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MOP-10584//Canadian Institutes of Health Research; MOP-49433//Canadian Institutes of Health Research; MOP-53217//Canadian Institutes of Health Research; MOP-62901//Canadian Institutes of Health Research; T0602145.02//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; 0/Dietary Fats; 0/Fatty Acids, Nonesterified; 0/RNA, Messenger; 0/Sterol Regulatory Element Binding Protein 1; 0/Triglycerides; 0/Wbscr14 protein, rat; EC 1.14.19.1/Stearoyl-CoA Desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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