Document Detail


Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle.
MedLine Citation:
PMID:  15388779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the effect of a single exercise session on the protein and mRNA expression of the monocarboxylate transporters MCT1 and MCT4 in rat soleus (SOL), and red (RG) and white gastrocnemius (WG) muscles. Muscle samples were obtained at rest before 2 h of treadmill exercise (21 m min(-1), 15% grade) and immediately after exercise, as well as 5, 10 and 24 h after exercise. During the 2 h exercise bout, MCT1 proteins in RG (+60%) and WG (+56%) were increased (P < 0.05). MCT1 protein was further increased thereafter, with peak increments occurring 10 h after exercise in RG (+157%), WG (+193%) and SOL (+179%) (P < 0.05). Twenty-four hours after exercise, MCT1 protein was still up-regulated in WG (+100%) and SOL (+55%) (P < 0.05), but not in RG. MCT1 mRNA was up-regulated during exercise in RG (+53%) and WG (+98%) and remained elevated until 24 h post-exercise in RG (P < 0.05), but in WG, MCT1 mRNA decreased transiently to pre-exercise levels at 5 and 10 h after exercise, before increasing again at 24 h (+150%) (P < 0.05). MCT4 protein and mRNA were not increased in WG muscle during and after exercise (P > 0.05). In contrast, during exercise, in RG (+41%) and SOL (+98%) MCT4 protein was increased (P < 0.05). Peak increases in MCT4 protein were observed 10 h after exercise in RG (+131%) and SOL (+323%) (P < 0.05). MCT4 protein was still up-regulated 24 h after exercise (RG: +106%; SOL +225%) (P < 0.05). MCT4 mRNA in RG was not increased until 10 (+132%) and 24 h after exercise (+55%) (P < 0.05). These studies have shown that MCT1 and 4 proteins are transiently up-regulated by a single bout of exercise, involving post-transcriptional and transcriptional mechanisms. Thus, MCT1 and MCT4 belong to a class of selected metabolic genes that are very rapidly up-regulated with an exercise stimulus.
Authors:
Lisa Coles; Jennifer Litt; Hideo Hatta; Arend Bonen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-23
Journal Detail:
Title:  The Journal of physiology     Volume:  561     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-04-06     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  253-61     Citation Subset:  IM    
Affiliation:
Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2l 3G1.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Female
Gene Expression / physiology
Monocarboxylic Acid Transporters / genetics*,  metabolism
Muscle Proteins / genetics*,  metabolism
Muscle, Skeletal / physiology*
Physical Exertion / physiology*
RNA, Messenger / analysis
Rats
Rats, Sprague-Dawley
Symporters / genetics*,  metabolism
Chemical
Reg. No./Substance:
0/Monocarboxylic Acid Transporters; 0/Mtc4 protein, rat; 0/Muscle Proteins; 0/RNA, Messenger; 0/Symporters; 0/monocarboxylate transport protein 1
Comments/Corrections

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