Document Detail


Exercise promotes alpha7 integrin gene transcription and protection of skeletal muscle.
MedLine Citation:
PMID:  18784336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The alpha7beta1 integrin is increased in skeletal muscle in response to injury-producing exercise, and transgenic overexpression of this integrin in mice protects against exercise-induced muscle damage. The present study investigates whether the increase in the alpha7beta1 integrin observed in wild-type mice in response to exercise is due to transcriptional regulation and examines whether mobilization of the integrin at the myotendinous junction (MTJ) is a key determinant in its protection against damage. A single bout of downhill running exercise selectively increased transcription of the alpha7 integrin gene in 5-wk-old wild-type mice 3 h postexercise, and an increased alpha7 chain was detected in muscle sarcolemma adjacent to tendinous tissue immediately following exercise. The alpha7B, but not alpha7A isoform, was found concentrated and colocalized with tenascin-C in muscle fibers lining the MTJ. To further validate the importance of the integrin in the protection against muscle damage following exercise, muscle injury was quantified in alpha7(-/-) mice. Muscle damage was extensive in alpha7(-/-) mice in response to both a single and repeated bouts of exercise and was largely restricted to areas of high MTJ concentration and high mechanical force near the Achilles tendon. These results suggest that exercise-induced muscle injury selectively increases transcription of the alpha7 integrin gene and promotes a rapid change in the alpha7beta integrin at the MTJ. These combined molecular and cellular alterations are likely responsible for integrin-mediated attenuation of exercise-induced muscle damage.
Authors:
Marni D Boppart; Sonja E Volker; Nicole Alexander; Dean J Burkin; Stephen J Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-09-10
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  295     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-04     Completed Date:  2009-01-06     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R1623-30     Citation Subset:  IM    
Affiliation:
Department of Kinesiology and Community Health, University of Illinois, Urbana, Illinois, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / biosynthesis*,  genetics
DNA, Complementary / biosynthesis,  genetics
Fluorescent Antibody Technique
Gene Expression / physiology*
Glyceraldehyde-3-Phosphate Dehydrogenases / physiology
Immunohistochemistry
Integrin alpha Chains / biosynthesis*,  genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle Fibers, Skeletal / pathology,  physiology
Muscle, Skeletal / physiology*
Physical Conditioning, Animal / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Running / physiology
Tenascin / physiology
Tendons / physiology
Grant Support
ID/Acronym/Agency:
P20-RR-018751/RR/NCRR NIH HHS; P20-RR-15581/RR/NCRR NIH HHS; R01-AG-014632/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/DNA, Complementary; 0/Integrin alpha Chains; 0/Tenascin; 0/integrin alpha7; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases
Comments/Corrections

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