| Exercise promotes alpha7 integrin gene transcription and protection of skeletal muscle. | |
| | |
MedLine Citation:
|
PMID: 18784336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The alpha7beta1 integrin is increased in skeletal muscle in response to injury-producing exercise, and transgenic overexpression of this integrin in mice protects against exercise-induced muscle damage. The present study investigates whether the increase in the alpha7beta1 integrin observed in wild-type mice in response to exercise is due to transcriptional regulation and examines whether mobilization of the integrin at the myotendinous junction (MTJ) is a key determinant in its protection against damage. A single bout of downhill running exercise selectively increased transcription of the alpha7 integrin gene in 5-wk-old wild-type mice 3 h postexercise, and an increased alpha7 chain was detected in muscle sarcolemma adjacent to tendinous tissue immediately following exercise. The alpha7B, but not alpha7A isoform, was found concentrated and colocalized with tenascin-C in muscle fibers lining the MTJ. To further validate the importance of the integrin in the protection against muscle damage following exercise, muscle injury was quantified in alpha7(-/-) mice. Muscle damage was extensive in alpha7(-/-) mice in response to both a single and repeated bouts of exercise and was largely restricted to areas of high MTJ concentration and high mechanical force near the Achilles tendon. These results suggest that exercise-induced muscle injury selectively increases transcription of the alpha7 integrin gene and promotes a rapid change in the alpha7beta integrin at the MTJ. These combined molecular and cellular alterations are likely responsible for integrin-mediated attenuation of exercise-induced muscle damage. |
| | |
Authors:
|
Marni D Boppart; Sonja E Volker; Nicole Alexander; Dean J Burkin; Stephen J Kaufman |
Related Documents
:
|
23097476 - The endurance athletes heart: acute stress and chronic adaptation. 12759116 - Noradrenergic and serotonergic blockade inhibits bdnf mrna activation following exercis... 11430586 - Neutrophil response to prolonged exercise in immune-competent and rag2/gamma c null mice. 10749846 - Single-unit recordings of arterial chemoreceptors from mouse petrosal ganglia in vitro. 2293816 - "recovery only" st-segment depression and the predictive accuracy of the exercise test. 8655446 - Feed deprivation of mares: plasma metabolite and hormonal concentrations and responses ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-09-10 |
Journal Detail:
|
Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 295 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2008 Nov |
Date Detail:
|
Created Date: 2008-11-04 Completed Date: 2009-01-06 Revised Date: 2013-06-05 |
Medline Journal Info:
|
Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: R1623-30 Citation Subset: IM |
Affiliation:
|
Department of Kinesiology and Community Health, University of Illinois, Urbana, Illinois, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD / biosynthesis*, genetics DNA, Complementary / biosynthesis, genetics Fluorescent Antibody Technique Gene Expression / physiology* Glyceraldehyde-3-Phosphate Dehydrogenases / physiology Immunohistochemistry Integrin alpha Chains / biosynthesis*, genetics Mice Mice, Inbred C57BL Mice, Knockout Muscle Fibers, Skeletal / pathology, physiology Muscle, Skeletal / physiology* Physical Conditioning, Animal / physiology* Reverse Transcriptase Polymerase Chain Reaction Running / physiology Tenascin / physiology Tendons / physiology |
| Grant Support | |
ID/Acronym/Agency:
|
P20-RR-018751/RR/NCRR NIH HHS; P20-RR-15581/RR/NCRR NIH HHS; R01-AG-014632/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD; 0/DNA, Complementary; 0/Integrin alpha Chains; 0/Tenascin; 0/integrin alpha7; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neutrophil-induced skeletal muscle damage: a calculated and controlled response following hindlimb u...
Next Document: Water deprivation increases Fos expression in hypothalamic corticotropin-releasing factor neurons in...