Document Detail


Exercise is the primary factor associated with Hsp70 induction in muscle of treadmill running rats.
MedLine Citation:
PMID:  16866780     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The cytoprotective, inducible stress protein, Hsp70, increases in muscles of rodents subjected to strenuous treadmill running. Most treadmill running protocols employ negative reinforcement to encourage animals to exercise. As these stimuli may themselves activate stress responses, the present investigation was conducted to determine their contribution to the exercise-induced expression of Hsp70. METHODS: Twenty-one male Sprague-Dawley rats were randomly divided into three equal groups including an exercise group (EX), which ran on a treadmill at 30 m min(-1) for 60 min; a stimulation group (STIM), which was not allowed to run, but was stimulated with compressed air and mild electric shock concurrently with their exercising cohort; and a control group (CON), which was housed in the treadmill room during the exercise period. Animals were killed 24 h post-experiment and hearts (H), soleii (SOL) and white gastrocnemii (WG) were harvested and analysed for Hsp70 content (mean% +/- SEM of standard). RESULTS: Significant increases in Hsp70 (as a % of standard) were noted in H and WG (H = 77.4 +/- 8.5; WG = 93.9 +/- 18.4) of EX but not in STIM (H = 32.5 +/- 4.6; WG = 32.0 +/- 3.4) or CON (H = 20.5 +/- 3.7; WG = 32.4 +/- 7.4). In SOL, Hsp70 expression in EX (126.7 +/- 6.2) was different from STIM (98.3 +/- 10.9) only. This occurred, despite the fact that all groups were exposed to a stressful environment and exhibited elevated (P < 0.001) temperatures (EX -41.2 +/- 0.1 degrees C > STIM -40.5 +/- 0.2 degrees C > CON -39.0 +/- 0.1 degrees C) indicative of a general stress response. CONCLUSIONS: These data suggest that exercise per se, rather than environmental conditions or noxious stimuli, are responsible for the induction of Hsp70 in rat muscle during treadmill running.
Authors:
E G Noble; R Ho; T Dzialoszynski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  187     ISSN:  1748-1708     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2007-03-29     Revised Date:  2009-02-03    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  495-501     Citation Subset:  IM    
Affiliation:
Faculty of Health Sciences, School of Kinesiology, The University of Western Ontario, London, ON, Canada. enoble@uwo.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Body Temperature / physiology
Body Weight / physiology
Electric Stimulation
Electrophoresis, Polyacrylamide Gel
Exercise Test
HSP40 Heat-Shock Proteins / biosynthesis*
Male
Muscle, Skeletal / metabolism*
Myocardium / metabolism
Physical Exertion / physiology*
Physical Stimulation / methods
Rats
Rats, Sprague-Dawley
Stress, Physiological / metabolism
Chemical
Reg. No./Substance:
0/HSP40 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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