Document Detail


Exercise intolerance resulting from a muscle-restricted mutation in the mitochondrial tRNA(Leu (CUN)) gene.
MedLine Citation:
PMID:  11680798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Some patients presenting with isolated lifelong exercise intolerance and ragged-red fibres, harbour skeletal-muscle restricted mutations in their mitochondrial DNA. AIM: To identify the molecular defect in a patient presenting with lifelong exercise intolerance, ragged-red fibres and deficiencies of complexes III and IV in skeletal muscle. METHODS: The muscle biopsy was studied for activities of the respiratory chain, histochemical stains, and sequencing the tRNA genes of mitochondrial DNA. RESULTS: The patient had a heteroplasmic mutation in the tRNA(Leu (CUN)) gene of mitochondrial DNA (G12334A). Clinical and morphological data as well as restriction fragment length polymorphism (RFLP) and single-fibre polymerase chain reaction (PCR) analyses strongly indicate that this molecular defect is the primary cause of the myopathy. CONCLUSION: Mutations in any mitochondrial gene should be considered in the differential diagnosis of patients with lifelong exercise intolerance, even when the neurological examination is normal.
Authors:
C Vives-Bauza; J Gamez; M Roig; P Briones; C Cervera; A Solano; J Montoya; A L Andreu
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Annals of medicine     Volume:  33     ISSN:  0785-3890     ISO Abbreviation:  Ann. Med.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-26     Completed Date:  2002-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8906388     Medline TA:  Ann Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  493-6     Citation Subset:  IM    
Affiliation:
Research Centre for Biochemistry and Molecular Biology, Universitary Hospital Vall d'Hebron, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Biopsy, Needle
Diagnosis, Differential
Exercise Tolerance / genetics*
Fatigue Syndrome, Chronic / diagnosis*,  genetics*
Female
Follow-Up Studies
Humans
Immunohistochemistry
Mitochondrial Myopathies / diagnosis*,  genetics*
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
RNA, Transfer / genetics*
Chemical
Reg. No./Substance:
9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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