Document Detail


Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid.
MedLine Citation:
PMID:  17543547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: (1) To investigate the effects of exercise and osteochondral (OC) injury on synovial fluid (SF) chondroitin sulfate (CS) and hyaluronan (HA) concentration and chain length, (2) to compare SF and cartilage CS data from joints with OC fragmentation, and (3) to compare SF CS and HA profiles with those seen in serum from the same horses. METHODS: Serum and SF were obtained from (1) normal horses after 8 weeks rest, (2) the same horses after 9 months treadmill training, and (3) horses with OC injury from racing. Articular cartilage was also collected from group 3 horses. Concentrations and chain lengths of CS and HA were determined by gel chromatography and fluorophore-assisted carbohydrate electrophoresis. RESULTS: SF CS peak chain length in the OC injury group increased significantly (18.7kDa) when compared to rested horses (11.6kDa), with exercise producing an intermediate chain length (15.6kDa). Cartilage and serum from the OC injury group had the abnormally long CS chains seen in SF from these horses. Total SF HA was significantly lower in the OC injury group compared to the rested group. Both the OC injury group and the exercised group had significant decreases in SF HA chain length compared to the rested group. CONCLUSIONS: Chain length of SF CS was increased by exercise and OC injury. Exercise resulted in a modest increase, whereas OC injury caused a marked increase. In contrast to CS, SF HA chain length was decreased by OC injury, and to a lesser extent by exercise. Chain length analysis of SF CS and HA may provide a useful tool for evaluation of joint health.
Authors:
M P Brown; T N Trumble; A H K Plaas; J D Sandy; M Romano; J Hernandez; K A Merritt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-31
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  15     ISSN:  1063-4584     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-09     Completed Date:  2008-04-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  England    
Other Details:
Languages:  eng     Pagination:  1318-25     Citation Subset:  IM    
Affiliation:
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-1432, USA. brownmu@mail.vetmed.ufl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / injuries,  metabolism*
Chondroitin Sulfates / blood,  chemistry*,  metabolism
Chromatography, Gel
Electrophoresis
Horses
Hyaluronic Acid / chemistry*,  metabolism
Physical Conditioning, Animal / physiology*
Synovial Fluid / metabolism,  physiology*
Chemical
Reg. No./Substance:
9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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