| Exercise induces hepatosplanchnic release of heat shock protein 72 in humans. | |
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MedLine Citation:
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PMID: 12411538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Physical exercise results in the appearance of heat shock protein (HSP) 72 in the circulation that precedes any increase in gene or protein expression in contracting skeletal muscle. In rodents, exercise increases liver HSP72 expression and the hepatosplanchnic viscera are known to release many acute phase proteins. In the present study, we tested the hypothesis that the splanchnic tissue beds release HSP72 during exercise. Seven male subjects performed 120 min of semi-recumbent cycling at 62 +/- 2 % of maximal oxygen uptake. Blood samples were obtained simultaneously from a brachial artery, a femoral vein and the hepatic vein prior to and at 30, 60 and 120 min of exercise. Leg blood flow (LBF) was measured by thermodilution in the femoral vein, and hepatosplanchnic blood flow (HBL) was measured using indocyanine green dye. Net leg and net hepatosplanchnic HSP72 balance were calculated as the product of LBF and femoral venous-arterial HSP72 difference and the product of HBF and hepatic venous-arterial HSP72 difference, respectively. Arterial plasma HSP72 was only detected in one subject at rest but progressively appeared in the arterial samples throughout exercise such that at 120 min it was detected in all subjects (0.88 +/- 0.35 pg l(-1); P < 0.05 compared with rest). The contracting muscle did not, however, contribute to this increase since there was no difference in the femoral venous-arterial HSP72 concentration at any time. Rather, the increase in arterial HSP72 was accounted for, at least in part, by release from the hepatosplanchnic viscera with values increasing (P < 0.05) from undetectable levels at rest to 5.2 +/- 0.2 pg min(-1) after 120 min. These data demonstrate that the splanchnic tissues release HSP72 during exercise and this release is responsible, in part, for the elevated systemic concentration of this protein during exercise. |
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Authors:
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Mark A Febbraio; Peter Ott; Henning Bay Nielsen; Adam Steensberg; Charlotte Keller; Peter Krustrup; Niels H Secher; Bente Klarlund Pedersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of physiology Volume: 544 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-11-04 Completed Date: 2003-05-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 957-62 Citation Subset: IM |
Affiliation:
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Copenhagen Muscle Research Centre, University of Copenhagen, Denmark. mark.febbraio@rmit.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bicycling Brachial Artery Exercise / physiology* Femoral Artery Femoral Vein HSP72 Heat-Shock Proteins Heat-Shock Proteins / blood, metabolism* Hepatic Veins Humans Leg / blood supply Liver / metabolism* Male Osmolar Concentration Regional Blood Flow Time Factors Viscera / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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