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Exercise induced regulation of muscular Na+,K+ pump, FXYD1 and NHE1 mRNA and protein expression - importance of training status, intensity and muscle type.
MedLine Citation:
PMID:  21325644     Owner:  NLM     Status:  Publisher    
It is investigated if exercise induced mRNA changes cause similar protein expression changes of Na(+), K(+) pump isoforms (α1, α2, β1, β2), FXYD1 and NHE1 in rat skeletal muscle. Expression was evaluated (n=8 per group) in Soleus and EDL after 1 day, 3 days and 3 weeks (5 sessions per week) of either sprint (4 x 3 min sprint + 1 min rest) or endurance (20 min) running. Two hours after exercise on day 1, no change in protein expression was apparent in either training group or muscle, whereas sprint exercise increased the mRNA of Soleus α2 (4.9±0.8 fold; P<0.05), β2 (13.2±4.4 fold; P<0.001) and NHE1 (12.0±3.1 fold; P<0.01). Two hours after sprint exercise, protein expression normalized to control samples was higher on day 3 than day 1 for Soleus α1 (41±18% increase vs. 15±8% reduction; P<0.05), α2 (64±35% increase vs. 37±12% reduction; P<0.05), β1 (17±21% increase vs. 14±29% reduction; P<0.05) and FXYD1 (35±16% increase vs. 13±10% reduction; P<0.05). In contrast, on day 3, Soleus α1 (0.1±0.1 fold; P<0.001), α2 (0.2±0.1 fold; P<0.001), β1 (0.4±0.1 fold; P<0.05) and β2 (2.9±1.7 fold; P<0.001) mRNA expression was lower than after exercise on day 1. After 3 weeks of training, no change in protein expression relative to control existed. In conclusion, increased expression of Na(+), K(+) pump subunits, FXYD1 and NHE1 after 3 days exercise training does not appear to be an effect of increased constitutive mRNA levels. Importantly, sprint exercise can reduce mRNA expression concomitant with increased protein expression.
Martin Krøyer Rasmussen; Carsten Juel; Nikolai Baastrup Nordsborg
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-16
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  -     ISSN:  1522-1490     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1University of Copenhagen.
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