Document Detail

Exercise-induced metallothionein expression in human skeletal muscle fibres.
MedLine Citation:
PMID:  15640275     Owner:  NLM     Status:  MEDLINE    
Exercise induces free oxygen radicals that cause oxidative stress, and metallothioneins (MTs) are increased in states of oxidative stress and possess anti-apoptotic effects. We therefore studied expression of the antioxidant factors metallothionein I and II (MT-I + II) in muscle biopsies obtained in response to 3 h of bicycle exercise performed by healthy men and in resting controls. Both MT-I + II proteins and MT-II mRNA expression increased significantly in both type I and II muscle fibres after exercise. Moreover, 24 h after exercise the levels of MT-II mRNA and MT-I + II proteins were still highly increased and the MT-II mRNA expression reached a 15-fold increase. As expected, immunohistochemical detection of malondialdehyde (MDA) and nitrotyrosine (NITT) showed that formation of free radicals and oxidative stress were clearly increased in exercising muscle peaking shortly after the end of exercise in both type I and II muscle fibres. This is the first report demonstrating that MT-I + II are significantly induced in human skeletal muscle fibres following exercise. As MT-I + II are antioxidant factors that protect various tissues during pathological conditions, the MT-I + II increases post exercise may represent a mechanism whereby contracting muscle fibres are protected against cellular stress and injury.
Milena Penkowa; Pernille Keller; Charlotte Keller; Juan Hidalgo; Mercedes Giralt; Bente Klarlund Pedersen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication     Date:  2005-01-07
Journal Detail:
Title:  Experimental physiology     Volume:  90     ISSN:  0958-0670     ISO Abbreviation:  Exp. Physiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-09-21     Revised Date:  2012-01-18    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  477-86     Citation Subset:  IM    
Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen, Denmark.
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MeSH Terms
Exercise / physiology*
Exercise Test
Metallothionein / biosynthesis*
Muscle Fibers, Skeletal / metabolism,  physiology*
Muscle Proteins / biosynthesis,  genetics
Muscle, Skeletal / metabolism,  physiology*
Oxidative Stress / physiology
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Muscle Proteins; 0/RNA, Messenger; 9038-94-2/Metallothionein
Retraction In:
Exp Physiol. 2011 Aug;96(8):816   [PMID:  21784791 ]

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