Document Detail


Exercise-induced increases in inflammatory cytokines in muscle and blood of horses.
MedLine Citation:
PMID:  21059019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
REASONS FOR PERFORMING STUDY: Studies have demonstrated increases in mRNA expression for inflammatory cytokines following exercise in horses and have suggested those markers of inflammation may play a role in delayed onset muscle soreness. However, measurement of mRNA expression in white blood cells is an indirect method. No studies to date have documented the cytokine response to exercise directly in muscle in horses.
HYPOTHESIS: This study tested the hypothesis that exercise increases cytokine markers of inflammation in blood and muscle.
METHODS: Blood and muscle biopsies were obtained from 4 healthy, unfit Standardbred mares (∼ 500 kg). The randomised crossover experiment was performed with the investigators performing the analysis blind to the treatment. Each horse underwent either incremental exercise test (GXT) or standing parallel control with the trials performed one month apart. During the GXT horses ran on a treadmill (1 m/s increases each min until fatigue, 6% grade). Blood and muscle biopsies were obtained 30 min before exercise, immediately after exercise and at 0.5, 1, 2, 6 and 24 h post GXT or at matched time points during the parallel control trials. Samples were analysed using real time-PCR for measurement of mRNA expression of interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 (IL-1). Data were analysed using t tests with the null hypothesis rejected when P < 0.10.
RESULTS: There were no changes (P > 0.10) in IL-1, IL-6, IFN-gamma or TNF-alpha during control. Exercise induced significant increases in IFN-gamma, IL1 and TNF-alpha in blood and significant increases in IFN-gamma, IL-6 and TNF-alpha in muscle. There were no significant changes in mRNA expression of IL-1 in muscle or IL-6 in blood following the GXT. These cytokine markers of inflammation all returned to preGXT levels by 24 h post GXT.
CONCLUSION: High intensity exercise results in a transient increase in the expression of inflammatory cytokines in muscle and blood.
Authors:
N R Liburt; A A Adams; A Betancourt; D W Horohov; K H McKeever
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Equine veterinary journal. Supplement     Volume:  -     ISSN:  -     ISO Abbreviation:  Equine Vet J Suppl     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-09     Completed Date:  2011-08-17     Revised Date:  2012-06-13    
Medline Journal Info:
Nlm Unique ID:  9614088     Medline TA:  Equine Vet J Suppl     Country:  England    
Other Details:
Languages:  eng     Pagination:  280-8     Citation Subset:  IM    
Copyright Information:
© 2010 EVJ Ltd.
Affiliation:
Department of Animal Sciences, Equine Science Center, Rutgers State University of New Jersey, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cross-Over Studies
Cytokines / genetics,  metabolism*
Female
Gene Expression Regulation / physiology*
Horse Diseases / blood,  metabolism*
Horses
Inflammation / blood,  metabolism,  veterinary*
Muscle, Skeletal / metabolism*
Muscular Diseases / blood,  metabolism,  veterinary
Pain / blood,  metabolism,  veterinary
Physical Conditioning, Animal / adverse effects*
RNA, Messenger / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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