| Exercise-induced changes in plasma composition increase erythrocyte Na+,K+-ATPase, but not Na+-K+-2Cl- cotransporter, activity to stimulate net and unidirectional K+ transport in humans. | |
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MedLine Citation:
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PMID: 14528028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We tested the hypothesis that exercise-induced changes in plasma composition result in peak stimulation of erythrocyte unidirectional K+ (JK,in) and net K+ (JK,net) transport within the first 120 s. In experimental series 1 (7 men; 2 women), plasma [K+] was continuously measured in vitro (37 degrees C) after the addition of red blood cells (RBCs) obtained from rested subjects (resting RBCs) into an exercise-simulated plasma (ESP; increased plasma osmolality, [K+], [H+], [lactate] and [adrenaline] (epinephrine)), and JK,net calculated. In experimental series 2 (7 men; 4 women), resting RBCs were incubated in true exercise plasma (TEP) obtained after two 30 s bouts of high intensity leg cycling exercise to determine JK,net and JK,in (via RBC 86Rb accumulation). JK,net of resting RBCs increased from 0.9 +/- 28.7 in resting plasma to 285 +/- 164 mmol (l RBCs)-1 h-1 in ESP and to 178 +/- 60 mmol (l RBCs)-1 h-1 after 10 s in TEP. Both JK,net and JK,in peaked within 10 s of incubation and decreased rapidly during the initial 120 s. The use of inhibitors for the Na+,K+-ATPase (ouabain) and the Na+-K+-2Cl- cotransporter (NKCC; bumetanide) indicated that rapid increases in JK,in and JK,net upon incubation of resting RBCs in TEP were due primarily to increased Na+,K+-ATPase activity; the NKCC appeared to be involved only when the Na+,K+-ATPase was blocked. It is concluded that RBCs rapidly increase JK,in and JK,net in response to exercise-induced changes in plasma composition. |
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Authors:
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Michael I Lindinger; Simon P Grudzien |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-10-03 |
Journal Detail:
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Title: The Journal of physiology Volume: 553 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-08-31 Revised Date: 2013-06-09 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 987-97 Citation Subset: IM |
Affiliation:
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Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada, N1G 2W1. mlinding@uoguelph.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Body Weight Bumetanide / pharmacology Erythrocytes / drug effects, metabolism* Exercise / physiology* Female Hematocrit Humans Kinetics Male Ouabain / pharmacology Potassium / blood* Sodium-Potassium-Chloride Symporters / blood* Sodium-Potassium-Exchanging ATPase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Sodium-Potassium-Chloride Symporters; 28395-03-1/Bumetanide; 630-60-4/Ouabain; 7440-09-7/Potassium; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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