Document Detail


Exercise-induced, but not creatine-induced, decrease in intramyocellular lipid content improves insulin sensitivity in rats.
MedLine Citation:
PMID:  21333514     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effect of creatine supplementation, alone or in combination with exercise training, on insulin sensitivity, intramyocellular lipid content (IMCL) and fatty acid translocase (FAT)/CD36 content was investigated in rats fed a sucrose-rich cafeteria diet during 12 weeks. Five experimental conditions were CON, receiving normal pellets; CAF, fed the cafeteria diet; CAF(TR), fed the cafeteria diet together with exercise training in weeks 8-12 and CAF(CR) and CAF(CRT) that were analogous to CAF and CAF(TR), respectively, but which received daily 2.5% of creatine monohydrate. During intravenous glucose tolerance test, compared with CON, whole-body glucose tolerance was reduced in CAF and CAF(CR) but not in CAF(TR) and CAF(CRT). Insulin-stimulated glucose transport in perfused red gastrocnemius muscles was impaired in CAF and CAF(CR) but not in the trained groups. IMCL content in soleus and extensor digitorum longus muscles was higher in CAF than in CON, but not in CAF(TR), CAF(CR) and CAF(CRT). Compared with CON and CAF, FAT/CD36 protein content in m. soleus, was ∼40% lower in CAF(CR), CAF(TR) and CAF(CRT). The fraction of fecal fat, as determined in a 3-week post hoc study, was 25% higher in CAF(CR) than in CON. Moreover, in CAF(CR), triglyceride concentration in blood and liver were significantly lower than in CAF. It is concluded that creatine supplementation in rats on a cafeteria diet inhibits IMCL accumulation via inhibition of gastrointestinal lipid absorption together with lower muscle FAT/CD36 content. Furthermore, exercise-induced but not creatine-induced reduction of IMCL is associated with improved insulin action on glucose transport in muscle cells.
Authors:
Morad Vaisy; Karolina Szlufcik; Katrien De Bock; Bert O Eijnde; Karen Van Proeyen; Kristin Verbeke; Paul Van Veldhoven; Peter Hespel
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-16
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  -     ISSN:  1873-4847     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Faculty of Kinesiology and Rehabilitation Sciences, Department of Biomedical Kinesiology, Research Centre for Exercise and Health, K.U.Leuven, Tervuursevest 101, B-3001 Leuven, Belgium.
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