Document Detail

Exercise-induced apoptosis of rat skeletal muscle and the effect of meloxicam.
MedLine Citation:
PMID:  11843167     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to evaluate the effect of exercise on apoptosis in rat gastrocnemius and soleus muscle tissue and to determine the effect of meloxicam, a novel non-steroidal anti-inflammatory drug (NSAID), on the ratio of exercise-induced apoptosis. Forty male Wistar rats were used in the experiments. Spontaneous wheel-running was used as an exercise protocol. Rats were divided randomly into four groups. Group A (n = 10) was the control group, in which rats did not perform any exercise. In group B (n = 10), gastrocnemius and soleus muscles were biopsied immediately after exercise. The rats in group C (n = 10) were placed back in their cages after exercise and allowed to rest for 48 h, after which the gastrocnemius and soleus muscles were biopsied. In group D (n = 10), rats were given 11 mg meloxicam (Mobic, Boehringer Ingelheim) per kilogram body weight per day p.o. for 2 days, after which gastrocnemius and soleus muscles were biopsied 48 h after exercise. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labelling (TUNEL) technique was used to detect DNA fragmentation in situ. TUNEL-positive nuclei were identified and counted. The apoptosis ratio in gastrocnemius muscle was 0.50x10(-3)+/-0.96x10(-3) in group A, 5.42x10(-3)+/-3.58x10(-3) in group B, 3.55x10(-3)+/-3.23x10(-3) in Group C and 3.52x10(-3)+/-1.00 in Group D; the ratios in soleus muscle were 0.98x10(-3)+/-1.83x10(-3), 3.03x10(-3)+/-2.78x10(-3), 4.48x10(-3)+/-3.32x10(-3) and 2.91x10(-3) 1.98x10(-3), respectively. The differences between the apoptosis ratios in group A and B, Group A and C, and Group A and D were statistically significant (P < 0.05). There was no statistically significant difference between group C and D. In conclusion, exercise increased apoptosis in gastrocnemius and soleus muscle tissue, and the apoptosis ratios were not affected by meloxicam.
Sule Arslan; Sevim Erdem; Aysen Sivri; Zafer Hasçelik; Ersin Tan
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Rheumatology international     Volume:  21     ISSN:  0172-8172     ISO Abbreviation:  Rheumatol. Int.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-14     Completed Date:  2002-08-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206885     Medline TA:  Rheumatol Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  133-6     Citation Subset:  IM    
Department of Physical Medicine and Rehabilitation, Hacettepe University, School of Medicine, Sihhiye, Ankara, Turkey.
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MeSH Terms
Analysis of Variance
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Apoptosis / drug effects*
Culture Techniques
Disease Models, Animal
Muscle, Skeletal / drug effects*,  pathology*
Physical Conditioning, Animal / adverse effects*
Random Allocation
Rats, Wistar
Reference Values
Sensitivity and Specificity
Statistics, Nonparametric
Thiazines / pharmacology*
Thiazoles / pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Thiazines; 0/Thiazoles; 71125-38-7/meloxicam

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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