| Exercise-induced Pyruvate Dehydrogenase Activation Is Not Affected by Seven Days of Bed Rest. | |
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MedLine Citation:
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PMID: 21680880 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), 6 healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an OGTT and a one-legged knee extensor exercise bout (45min at 60% Wmax) with muscle biopsies obtained from vastus lateralis before, immediately after exercise, and at 3h of recovery. Blood samples were taken from the femoral vein and artery before and after 40min of exercise. Glucose intake elicited a larger (P≤0.05) insulin response after bed rest than before, indicating glucose intolerance. There were no differences in lactate release/uptake across the exercising muscle before and after bed rest, but glucose uptake after 40min of exercise was larger (P≤0.05) before bed rest than after. Muscle glycogen content tended to be higher (0.05<P≤0.10) after bed rest than before, but muscle glycogen breakdown in response to exercise was similar before and after bed rest. PDH-E1α protein content did not change in response to bed rest or in response to the exercise intervention. Exercise increased (P≤0.05) the activity of PDH in the active form (PDHa) and induced (P≤0.05) dephosphorylation of PDH-E1α on Ser293, ser295 and Ser300, with no difference before and after bed rest. In conclusion, although 7 days of bed rest induced whole body glucose intolerance, exercise-induced PDH regulation in skeletal muscle was not changed. This suggests that exercise-induced PDH regulation in skeletal muscle is maintained in glucose intolerant (e.g. insulin resistant) individuals. |
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Authors:
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Kristian Kiilerich; Stine Ringholm; Rasmus S Biensø; James P Fisher; Ninna Iversen; Gerrit Van Hall; Jorgen F P Wojtaszewski; Bengt Saltin; Carsten Lundby; Jose A L Calbet; Henriette Pilegaard |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-16 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Copenhagen. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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