Document Detail

Exercise directly enhances myocardial tolerance to ischaemia-reperfusion injury in the rat through a protein kinase C mediated mechanism.
MedLine Citation:
PMID:  11179278     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether exercise is capable of protecting the myocardium from experimental infarction and to explore the involvement of protein kinase C, a key signalling protein, in the development of any protection observed. METHODS: Rats were exercised on a treadmill for 30 minutes at 23-27 m/min. Sham treated animals were placed on the stationary treadmill but not exercised. Twenty four hours later, hearts were Langendorff perfused and subjected to 35 minute left main coronary artery occlusion followed by 120 minute reperfusion. Infarct size was determined by tetrazolium staining and expressed as a percentage of the risk zone (I/R%). To examine the potential signalling pathway, animals were treated with either the selective protein kinase C inhibitor chelerythrine, 5 mg/kg intraperitoneally, or with vehicle 10 minutes before the exercise or sham treadmill period. RESULTS: In the non-exercised group, mean (SEM) I/R was 48.4 (3.0)%. In the exercised group, infarct size was reduced to 17.3 (3.0)% (p < 0.01). Infarct size limitation induced by exercise was abolished by chelerythrine (I/R 45.0 (6.0)%). Chelerythrine pretreatment alone did not have any effect on infarct size (I/R 51.1 (3.9)%). Differences in infarct size were independent of risk zone size and myocardial contractile function during ischaemia-reperfusion. CONCLUSIONS: Experimental moderate exercise induces protection against myocardial infarction 24 hours later. Protein kinase C activation during exercise appears to be an important signal mediator of this protective response.
N Yamashita; G F Baxter; D M Yellon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Heart (British Cardiac Society)     Volume:  85     ISSN:  1468-201X     ISO Abbreviation:  Heart     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9602087     Medline TA:  Heart     Country:  England    
Other Details:
Languages:  eng     Pagination:  331-6     Citation Subset:  IM    
Hatter Institute and Centre for Cardiology, UCL Hospitals and Medical School, Grafton Way, London WC1E 6DB, UK.
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MeSH Terms
Myocardial Infarction / pathology,  physiopathology,  prevention & control*
Myocardial Reperfusion Injury / physiopathology*
Phenanthridines / pharmacology
Physical Conditioning, Animal
Physical Exertion / physiology*
Protein Kinase C / antagonists & inhibitors,  physiology*
Signal Transduction
Reg. No./Substance:
0/Alkaloids; 0/Benzophenanthridines; 0/Phenanthridines; 34316-15-9/chelerythrine; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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