Document Detail


Exercise causes oxidative damage to rat skeletal muscle microsomes while increasing cellular sulfhydryls.
MedLine Citation:
PMID:  8289576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The physiological and biochemical demands on contracting muscle make this tissue particularly susceptible to molecular and cellular damage. We looked at membrane structures in cardiac and skeletal muscle and in erythrocytes for exercise-induced lipid peroxidation. These tissues were removed from each of the rats used in this study. We also examined and compared the effects of exercise on the redox status of blood plasma, erythrocytes and cardiac and skeletal muscle from the same rats. We used a swim stress protocol to exercise the rats to exhaustion. Some form of chemical modification or oxidative damage to membranes was observed in all of the tissues tested. Cardiac muscle microsomes from exercised rats exhibited increased malondialdehyde and decreased phospholipid (control, 249.1 vs exercised, 120.6 nmols phospholipid/mg protein). Skeletal muscle microsomes showed decreased sulfhydryls, decreased phospholipid (control, 1,276.9 vs exercised, 137.7 nmols phospholipid/mg protein), increased malondialdehyde and greater protein crosslinking after exercise. Erythrocyte membranes also exhibited exercised-induced protein oxidation. However, the total cellular sulfhydryl content remained the same in erythrocytes and cardiac tissue but increased in blood plasma (control, 10.8 vs exercised, 24.7 mumols SH/dl plasma) and skeletal muscle after exercise. We conclude that exercise profoundly effects membrane structures. The body compensates for this lipid peroxidation and protein damage by increasing total cellular sulfhydryls in blood plasma and skeletal muscle which would aid in repair of the damaged membranes.
Authors:
S U Rajguru; G S Yeargans; N W Seidler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  54     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1994  
Date Detail:
Created Date:  1994-02-18     Completed Date:  1994-02-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  149-57     Citation Subset:  IM; S    
Affiliation:
Department of Biochemistry, University of Health Sciences, College of Osteopathic Medicine, Kansas City 64124.
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MeSH Terms
Descriptor/Qualifier:
Animals
Erythrocyte Membrane / metabolism
Erythrocytes / metabolism
Heart / physiology*
Male
Membranes / metabolism
Microsomes / metabolism*
Muscles / metabolism*,  physiology*
Myocardium / metabolism*
Oxidation-Reduction
Physical Exertion / physiology*
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism*,  toxicity*
Sulfhydryl Compounds / metabolism*
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Sulfhydryl Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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