Document Detail


Exercise attenuates matrix metalloproteinase activity in preexisting atherosclerotic plaque.
MedLine Citation:
PMID:  21334624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Few studies have investigated if exercise by itself has anti-atherosclerotic effects, without combining interventions with a low-fat diet. We studied the effects of exercise as a stand-alone intervention on preexisting atheromata by measuring not only plaque size but also the levels of plaque-destabilizing matrix-metalloproteinase (MMP) activity in vivo.
METHODS AND RESULTS: We used near-infrared fluorescent (NIRF) molecular imaging with an MMP-2/9 activatable NIRF probe to visualize the inflammatory protease activity within preexisting atheromata of 17-week-old ApoE(-/-) mice on: (a) normal chow diet (NCD), (b) Western diet (WD), and (c) WD with treadmill exercise for 10 weeks. We also measured tissue levels of aortic lipid peroxidation (LPO) and plasma levels of glucose/lipid/cytokine profiles. Exercise did not attenuate growth of preexisting atheromatous plaques. However, exercise strongly decreased proteolytic activity in plaques for animals on WD, with levels decreasing almost to NCD levels. Exercise was associated with decreased aortic LPO levels and increased blood adiponectin/leptin levels; however, exercise did not affect WD-consumption/weight-gain or improve blood glucose/lipid profiles.
CONCLUSIONS: Exercise training reduced aortic MMP activity in mice with preexisting atheromata, even though they remained on a high fat diet and plaque-growth was not attenuated.
Authors:
Soo-Min Shon; Jong-Ho Park; Matthias Nahrendorf; Dawid Schellingerhout; Jeong-Yeon Kim; Byeong-Teck Kang; Sang-Wuk Jeong; Eo-Jin Kim; Ju Hee Ryu; Kwangmeyung Kim; Ick Chan Kwon; Dong Kun Lee; Myoung Mook Lee; Dong-Eog Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-02
Journal Detail:
Title:  Atherosclerosis     Volume:  216     ISSN:  1879-1484     ISO Abbreviation:  Atherosclerosis     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-06     Completed Date:  2011-08-23     Revised Date:  2012-04-09    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Molecular Imaging and Neurovascular Research Laboratory, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Adiponectin / blood
Analysis of Variance
Animals
Aorta / enzymology*,  pathology
Aortic Diseases / enzymology,  genetics,  pathology,  therapy*
Apolipoproteins E / deficiency,  genetics
Atherosclerosis / enzymology,  genetics,  pathology,  therapy*
Blood Glucose / metabolism
Body Weight
Cytokines / blood
Disease Models, Animal
Down-Regulation
Exercise Therapy*
Immunohistochemistry
Infrared Rays
Leptin / blood
Lipid Peroxidation
Lipids / blood
Matrix Metalloproteinase 2 / metabolism*
Matrix Metalloproteinase 9 / metabolism*
Mice
Mice, Knockout
Microscopy
Molecular Imaging / methods
Plaque, Atherosclerotic / enzymology,  genetics,  pathology,  therapy*
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Adipoq protein, mouse; 0/Apolipoproteins E; 0/Blood Glucose; 0/Cytokines; 0/Leptin; 0/Lipids; EC 3.4.24.-/Mmp9 protein, mouse; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.24/Mmp2 protein, mouse; EC 3.4.24.35/Matrix Metalloproteinase 9

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