| Exercise training and caloric restriction prevent reduction in cardiac Ca2+-handling protein profile in obese rats. | |
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MedLine Citation:
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PMID: 20644006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies show that exercise training and caloric restriction improve cardiac function in obesity. However, the molecular mechanisms underlying this effect on cardiac function remain unknown. Thus, we studied the effect of exercise training and/or caloric restriction on cardiac function and Ca(2+) handling protein expression in obese rats. To accomplish this goal, male rats fed with a high-fat and sucrose diet for 25 weeks were randomly assigned into 4 groups: high-fat and sucrose diet, high-fat and sucrose diet and exercise training, caloric restriction, and exercise training and caloric restriction. An additional lean group was studied. The study was conducted for 10 weeks. Cardiac function was evaluated by echocardiography and Ca(2+) handling protein expression by Western blotting. Our results showed that visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels were higher in rats on the high-fat and sucrose diet compared with the lean rats. Cardiac nitrate levels, reduced/oxidized glutathione, left ventricular fractional shortening, and protein expression of phosphorylated Ser(2808)-ryanodine receptor and Thr(17)-phospholamban were lower in rats on the high-fat and sucrose diet compared with lean rats. Exercise training and/or caloric restriction prevented increases in visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels and prevented reduction in cardiac nitrate levels and reduced:oxidized glutathione ratio. Exercise training and/or caloric restriction prevented reduction in left ventricular fractional shortening and in phosphorylation of the Ser(2808)-ryanodine receptor and Thr(17)-phospholamban. These findings show that exercise training and/or caloric restriction prevent cardiac dysfunction in high-fat and sucrose diet rats, which seems to be attributed to decreased circulating neurohormone levels. In addition, this nonpharmacological paradigm prevents a reduction in the Ser(2808)-ryanodine receptor and Thr(17)-phospholamban phosphorylation and redox status. |
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Authors:
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Ellena Christina Paulino; Julio Cesar Batista Ferreira; Luiz Roberto Bechara; Jeane Mike Tsutsui; Wilson Mathias; Fabio Bessa Lima; Dulce Elena Casarini; Antonio Carlos Cicogna; Patricia Chakur Brum; Carlos Eduardo Negrão |
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Publication Detail:
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Type: Journal Article Date: 2010-07-19 |
Journal Detail:
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Title: Hypertension Volume: 56 ISSN: 1524-4563 ISO Abbreviation: Hypertension Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-16 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7906255 Medline TA: Hypertension Country: United States |
Other Details:
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Languages: eng Pagination: 629-35 Citation Subset: IM |
Affiliation:
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Heart Institute InCor, University of Sa˜o Paulo Medical School, Sa˜o Paulo, Brazil. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology Blotting, Western Calcium / metabolism* Calcium-Binding Proteins / genetics, metabolism Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism Caloric Restriction* Cyclic AMP-Dependent Protein Kinases / metabolism Dietary Carbohydrates / administration & dosage, adverse effects Dietary Fats / administration & dosage, adverse effects Heart Rate / physiology Humans Male Myocardium / metabolism Obesity / etiology, prevention & control* Phosphorylation Physical Conditioning, Animal / physiology* Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Ryanodine Receptor Calcium Release Channel / genetics, metabolism Sarcoplasmic Reticulum Calcium-Transporting ATPases / genetics, metabolism Sucrose / administration & dosage, adverse effects Ventricular Function, Left / physiology |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Proteins; 0/Dietary Carbohydrates; 0/Dietary Fats; 0/Ryanodine Receptor Calcium Release Channel; 0/phospholamban; 57-50-1/Sucrose; 7440-70-2/Calcium; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2; EC 3.6.3.8/Atp2a2 protein, rat; EC 3.6.3.8/Sarcoplasmic Reticulum Calcium-Transporting ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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