| Exercise preconditioning reduces neuronal apoptosis in stroke by up-regulating heat shock protein-70 (heat shock protein-72) and extracellular-signal-regulated-kinase 1/2. | |
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MedLine Citation:
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PMID: 20083167 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise preconditioning induces neuroprotection after stroke. We investigated the beneficial role of heat shock protein-70 (HSP-70) and phosphorylated extracellular-signal-regulated-kinase 1/2 (pERK 1/2), as they pertain to reducing apoptosis and their influence on Bcl-x(L), Bax, and apoptosis-inducing factor (AIF) in rats subjected to ischemia and reperfusion. Adult male Sprague-Dawley rats were subjected to 30 min of exercise on a treadmill for 1, 2, or 3 weeks. Stroke was induced by a 2-h middle cerebral artery (MCA) occlusion using an intraluminal filament. Protein levels of HSP-70, pERK 1/2, Bcl-x(L), Bax, and AIF were analyzed using Western blot. Neuroprotection was based on levels of apoptosis (TUNEL) and infarct volume (Nissl staining). Immunocytochemistry was used for cellular expression of HSP-70 and pERK 1/2. Significant (P<0.05) up-regulation of HSP-70 and pERK 1/2 after 3 weeks of exercise coincided with significant (P<0.05) reduction in neuronal apoptosis and brain infarct volume. Inhibition of either one of these two factors showed a significant (P<0.05) reversal in the neuroprotection. Bax and AIF were down-regulated, while levels of Bcl-x(L) were up-regulated in response to stroke after exercise. Inhibiting HSP-70 or pERK 1/2 reversed this resultant increase or decrease. Our results indicate that exercise diminishes neuronal injury in stroke by up-regulating HSP-70 and ERK 1/2. |
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Authors:
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B Liebelt; P Papapetrou; A Ali; M Guo; X Ji; C Peng; R Rogers; A Curry; D Jimenez; Y Ding |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-18 |
Journal Detail:
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Title: Neuroscience Volume: 166 ISSN: 1873-7544 ISO Abbreviation: Neuroscience Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-05 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
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Languages: eng Pagination: 1091-100 Citation Subset: IM |
Copyright Information:
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Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Neurosurgery, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / physiology* Apoptosis Regulatory Proteins / metabolism Brain Infarction / physiopathology, prevention & control, therapy Cytoprotection / physiology Disease Models, Animal Exercise Therapy / methods HSP70 Heat-Shock Proteins / metabolism* Immunohistochemistry Infarction, Middle Cerebral Artery / metabolism, physiopathology, therapy Male Mitogen-Activated Protein Kinase 3 / metabolism* Nerve Degeneration / physiopathology, prevention & control, therapy* Physical Conditioning, Animal / physiology* Rats Rats, Sprague-Dawley Reperfusion Injury / metabolism, physiopathology, therapy Stroke / metabolism, physiopathology, therapy* Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/HSP70 Heat-Shock Proteins; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3 |
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