Document Detail

Exercise preconditioning reduces neuronal apoptosis in stroke by up-regulating heat shock protein-70 (heat shock protein-72) and extracellular-signal-regulated-kinase 1/2.
MedLine Citation:
PMID:  20083167     Owner:  NLM     Status:  MEDLINE    
Exercise preconditioning induces neuroprotection after stroke. We investigated the beneficial role of heat shock protein-70 (HSP-70) and phosphorylated extracellular-signal-regulated-kinase 1/2 (pERK 1/2), as they pertain to reducing apoptosis and their influence on Bcl-x(L), Bax, and apoptosis-inducing factor (AIF) in rats subjected to ischemia and reperfusion. Adult male Sprague-Dawley rats were subjected to 30 min of exercise on a treadmill for 1, 2, or 3 weeks. Stroke was induced by a 2-h middle cerebral artery (MCA) occlusion using an intraluminal filament. Protein levels of HSP-70, pERK 1/2, Bcl-x(L), Bax, and AIF were analyzed using Western blot. Neuroprotection was based on levels of apoptosis (TUNEL) and infarct volume (Nissl staining). Immunocytochemistry was used for cellular expression of HSP-70 and pERK 1/2. Significant (P<0.05) up-regulation of HSP-70 and pERK 1/2 after 3 weeks of exercise coincided with significant (P<0.05) reduction in neuronal apoptosis and brain infarct volume. Inhibition of either one of these two factors showed a significant (P<0.05) reversal in the neuroprotection. Bax and AIF were down-regulated, while levels of Bcl-x(L) were up-regulated in response to stroke after exercise. Inhibiting HSP-70 or pERK 1/2 reversed this resultant increase or decrease. Our results indicate that exercise diminishes neuronal injury in stroke by up-regulating HSP-70 and ERK 1/2.
B Liebelt; P Papapetrou; A Ali; M Guo; X Ji; C Peng; R Rogers; A Curry; D Jimenez; Y Ding
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-18
Journal Detail:
Title:  Neuroscience     Volume:  166     ISSN:  1873-7544     ISO Abbreviation:  Neuroscience     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-05     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1091-100     Citation Subset:  IM    
Copyright Information:
Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
Department of Neurosurgery, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
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MeSH Terms
Apoptosis / physiology*
Apoptosis Regulatory Proteins / metabolism
Brain Infarction / physiopathology,  prevention & control,  therapy
Cytoprotection / physiology
Disease Models, Animal
Exercise Therapy / methods
HSP70 Heat-Shock Proteins / metabolism*
Infarction, Middle Cerebral Artery / metabolism,  physiopathology,  therapy
Mitogen-Activated Protein Kinase 3 / metabolism*
Nerve Degeneration / physiopathology,  prevention & control,  therapy*
Physical Conditioning, Animal / physiology*
Rats, Sprague-Dawley
Reperfusion Injury / metabolism,  physiopathology,  therapy
Stroke / metabolism,  physiopathology,  therapy*
Up-Regulation / physiology
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/HSP70 Heat-Shock Proteins; EC Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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