| Exclusion of synaptotagmin V at the phagocytic cup by the Leishmania donovani lipophosphoglycan results in decreased promastigote internalization. | |
| | |
MedLine Citation:
|
PMID: 21680635 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Regulators of membrane fusion play an important role in phagocytosis as they regulate the focal delivery of endomembrane which is required for optimal internalization of large particles. During internalization of Leishmania promastigotes, the surface glycolipid lipophosphoglycan (LPG) is transferred to the macrophage membrane and modifies its fusogenic properties. In this study, we investigated the impact of LPG on the recruitment of the exocytosis regulator synaptotagmin V (Syt V) at the area of internalization and on the early steps of phagocytosis. Using L. donovani LPG-defective mutants and LPG-coated particles, we established that LPG reduces the phagocytic capacity of macrophages and we showed that it causes exclusion of Syt V from the nascent phagosome. Silencing of Syt V inhibited phagocytosis to the same extent than LPG, and these effects were not cumulative, consistent with a Syt V-dependent mechanism for the inhibition of phagocytosis by LPG. Previous work revealed that LPG-mediated exclusion of Syt V from phagosomes prevent recruitment of the vacuolar ATPase and acidification. Thus, whereas exclusion of Syt V from forming phagosomes may be beneficial for the creation of an hospitable intracellular niche, it reduces the phagocytic capacity of macrophages. We propose that the cost associated with a reduced internalization rate may be compensated by increased survival and could lead to a greater overall parasite fitness. |
| | |
Authors:
|
Adrien F Vinet; Silvana Jananji; Salvatore J Turco; Mitsunori Fukuda; Albert Descoteaux |
Related Documents
:
|
11350525 - Infection-mediated early-onset periodontal disease in p/e-selectin-deficient mice. 16498065 - Protective and destructive immunity in the periodontium: part 1--innate and humoral imm... 14996275 - Dentin matrix proteins and soluble factors: intrinsic regulatory signals for healing an... 22575995 - Lipopolysaccharide- and superantigen-modulated superoxide production and monocyte hypor... 10211465 - Caspases as treatment targets in stroke and neurodegenerative diseases. 9654325 - Molecular biology of septic shock. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-16 |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: - ISSN: 1465-2080 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
INRS- Institut Armand-Frappier; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A novel FK-506 binding like protein that lacks peptidyl-prolyl isomerase activity is involved in int...
Next Document: JmjN interacts with JmjC to ensure selective degradation of Gis1 by the proteasome.