| Excited-state dynamics of dGMP measured by steady-state and femtosecond fluorescence spectroscopy. | |
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MedLine Citation:
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PMID: 20085298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The room-temperature fluorescence of 2'-deoxyguanosine 5'-monophosphate (dGMP) in aqueous solution is studied by steady-state and time-resolved fluorescence spectroscopy. The steady-state fluorescence spectrum of dGMP shows one band centered at 334 nm but has an extraordinary long red tail, extending beyond 700 nm. Both the fluorescence quantum yield and the relative weight of the 334 nm peak increase with the excitation wavelength. The initial fluorescence anisotropy after excitation at 267 nm is lower than 0.2 for all emission wavelengths, indicating an ultrafast S(2) --> S(1) internal conversion. The fluorescence decays depend strongly on the emission wavelength, getting longer with the wavelength. A rise time of 100-150 fs was observed for wavelengths longer than 450 nm, in accordance with a gradual red shift of the time-resolved spectra. The results are discussed in terms of a relaxation occurring mainly on the lowest excited (1)pi pi*-state surface toward a conical intersection with the ground state, in line with recent theoretical predictions. Our results show that the excited-state population undergoes a substantial "spreading out" before reaching the CI, explaining the complex dynamics observed. |
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Authors:
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Francois-Alexandre Miannay; Thomas Gustavsson; Akos Banyasz; Dimitra Markovitsi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of physical chemistry. A Volume: 114 ISSN: 1520-5215 ISO Abbreviation: J Phys Chem A Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-04 Completed Date: 2010-05-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9890903 Medline TA: J Phys Chem A Country: United States |
Other Details:
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Languages: eng Pagination: 3256-63 Citation Subset: IM |
Affiliation:
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Laboratoire Francis Perrin, CEA/DSM/IRAMIS/SPAM-CNRS URA 2453, CEA Saclay, F-91191 Gif-sur-Yvette, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Deoxyguanine Nucleotides
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chemistry* Quantum Theory* Spectrometry, Fluorescence Time Factors Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Deoxyguanine Nucleotides; 7732-18-5/Water; 902-04-5/2'-deoxyguanosine 5'-phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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