Document Detail


Excitatory amino acid transporter 2 and excitatory amino acid transporter 1 negatively regulate calcium-dependent proliferation of hippocampal neural progenitor cells and are persistently upregulated after injury.
MedLine Citation:
PMID:  22092549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a transgenic mouse (Mus musculus) in which nestin-expressing progenitors are labeled with enhanced green fluorescent protein, we previously characterized the expression of excitatory amino acid transporter 2 (GltI) and excitatory amino acid transporter 1 (Glast) on early neural progenitors in vivo. To address their functional role in this cell population, we manipulated their expression in P7 neurospheres isolated from the dentate gyrus. We observed that knockdown of GltI or Glast was associated with decreased bromodeoxyuridine incorporation and neurosphere formation. Moreover, we determined that both glutamate transporters regulated progenitor proliferation in a calcium-dependent and metabotropic glutamate receptor-dependent manner. To address the relevance of this in vivo, we utilized models of acquired brain injury, which are known to induce hippocampal neurogenesis. We observed that GltI and Glast were specifically upregulated in progenitors following brain injury, and that this increased expression was maintained for many weeks. Additionally, we found that recurrently injured animals with increased expression of glutamate transporters within the progenitor population were resistant to subsequent injury-induced proliferation. These findings demonstrate that GltI and Glast negatively regulate calcium-dependent proliferation in vitro and that their upregulation after injury is associated with decreased proliferation after brain trauma.
Authors:
Jennifer A Gilley; Steven G Kernie
Related Documents :
17513659 - The antinociceptive effects of local injections of propofol in rats are mediated in par...
10189949 - Effects of aqueous extracts from quercus ilex l. root bark, punica granatum l. fruit pe...
10781889 - Reduction of gastrointestinal toxicity of nsaids via molecular modifications leading to...
21815409 - Evaluation of ent-kaurenoic acid derivatives for their anticariogenic activity.
3709479 - Methanol extract of grain dust shows complement fixing activity and other characteristi...
196069 - Antiproliferative activity of anti-inflammatory drugs in two mammalian cell culture lines.
18674899 - Synthesis and antiviral properties of some polyphenols related to salvia genus.
11950779 - Glucuronidation of statins in animals and humans: a novel mechanism of statin lactoniza...
14629169 - Diastereoselective synthesis of cyclopropane amino acids using diazo compounds generate...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-17
Journal Detail:
Title:  The European journal of neuroscience     Volume:  34     ISSN:  1460-9568     ISO Abbreviation:  Eur. J. Neurosci.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-29     Completed Date:  2012-04-04     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  8918110     Medline TA:  Eur J Neurosci     Country:  France    
Other Details:
Languages:  eng     Pagination:  1712-23     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Calcium / metabolism*
Cell Proliferation*
Cells, Cultured
Dentate Gyrus / cytology*,  injuries*,  physiology
Excitatory Amino Acid Antagonists / metabolism
Excitatory Amino Acid Transporter 1 / genetics,  metabolism*
Excitatory Amino Acid Transporter 2 / genetics,  metabolism*
Mice
Mice, Transgenic
Neural Stem Cells / cytology,  physiology*
RNA, Small Interfering / genetics,  metabolism
Xanthenes / metabolism
Grant Support
ID/Acronym/Agency:
R01 NS048192/NS/NINDS NIH HHS; R01 NS048192/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Excitatory Amino Acid Antagonists; 0/Excitatory Amino Acid Transporter 1; 0/Excitatory Amino Acid Transporter 2; 0/LY 341495; 0/RNA, Small Interfering; 0/Xanthenes; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Retention force of plastic clips on implant bars: a randomized controlled trial.
Next Document:  RELATIONSHIPS AMONG SUBGROSS ANATOMY, COMPUTED TOMOGRAPHY, AND HISTOLOGIC FINDINGS IN DOGS WITH DISE...