Document Detail

Excitatory amino acid-induced formation of inositol phosphates in guinea-pig cerebral cortical slices: involvement of ionotropic or metabotropic receptors?
MedLine Citation:
PMID:  1975837     Owner:  NLM     Status:  MEDLINE    
In cerebral cortical slices from the guinea-pig, quinoxalinedione derivatives antagonised the generation of 3H-inositol phosphates evoked by the excitatory amino acids quisqualate and DL-alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid but were without effect on the trans-DL-1-amino-1,3-cyclopentanedicarboxylic acid and L-glutamate responses. Omission of calcium from the medium reduced the accumulation of 3H-inositol phosphates induced by incubation with trans-DL-1-amino-1,3-cyclopentanedicarboxylic acid (incubation for 45 min) by greater than 50%, whereas the responses to L-glutamate and the two other amino acid analogues were reduced by approximately 20%. Generation of inositol 1,4,5-trisphosphate over a 30-s period by treatment with quisqualate, trans-DL-1-amino-1,3-cyclopentane-dicarboxylic acid, KCl, and carbachol was abolished in the presence of nominally calcium-free medium. L-Glutamate induced a large, rapid increase in inositol 1,4,5-trisphosphate mass (more than three-fold), which was, however, unaffected by omission of calcium from the medium. These results indicate that of the excitatory amino acids tested, only L-glutamate may be classed as a metabotropic receptor agonist in guinea-pig cerebral cortical slices with respect to generation of inositol phosphates. The other agents appear to stimulate accumulation of inositol phosphates, at least in part through some mechanism requiring the presence of extracellular Ca2+, presumably Ca2+ entry.
S P Alexander; S J Hill; D A Kendall
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  55     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-10-17     Completed Date:  1990-10-17     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1439-41     Citation Subset:  IM    
Department of Physiology and Pharmacology, University of Nottingham Medical School, Queen's Medical Centre, England.
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MeSH Terms
Amino Acids / pharmacology*
Calcium Chloride / pharmacology
Carbachol / pharmacology
Cerebral Cortex / drug effects,  metabolism*
Cycloleucine / pharmacology
Glutamates / pharmacology
Glutamic Acid
Ibotenic Acid / analogs & derivatives,  pharmacology*
Inositol 1,4,5-Trisphosphate / metabolism*
Oxadiazoles / pharmacology
Oxazoles / pharmacology*
Potassium Chloride / pharmacology
Quisqualic Acid
Receptors, Glutamate
Receptors, Neurotransmitter / drug effects,  physiology*
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Amino Acids; 0/Glutamates; 0/Oxadiazoles; 0/Oxazoles; 0/Receptors, Glutamate; 0/Receptors, Neurotransmitter; 10043-52-4/Calcium Chloride; 2552-55-8/Ibotenic Acid; 51-83-2/Carbachol; 52-52-8/Cycloleucine; 52809-07-1/Quisqualic Acid; 56-86-0/Glutamic Acid; 7447-40-7/Potassium Chloride; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; 85166-31-0/Inositol 1,4,5-Trisphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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