Document Detail

Excitatory amino acid concentrations increase in the spinal cord dorsal horn after repeated intramuscular injection of acidic saline.
MedLine Citation:
PMID:  16297556     Owner:  NLM     Status:  MEDLINE    
Chronic muscle pain is common and often difficult to treat. In this study, we further characterize a model of chronic muscle pain induced by repeated intramuscular injection of acidic saline. Two injections of acid into muscle separated by 5 days result in secondary mechanical hyperalgesia that lasts for up to 4 weeks. Blockade of spinal NMDA receptors prior to the second injection intramuscular acid injection delays the onset of hyperalgesia, where as the maintenance phase of hyperalgesia, evaluated 1 week after the second intramuscular injection, is dependent on activation of spinal AMPA/kainate and NMDA receptors. In order to determine if behavioral hyperalgesia and glutamate receptor involvement are associated with increased concentrations of excitatory amino acids (EAA), we utilized microdialysis to evaluate extracellular glutamate and aspartate concentrations in the spinal dorsal horn during the first and second intramuscular acid injections, and 1 week after the development of mechanical hyperalgesia. The second intramuscular injection evoked a calcium-dependent increase in both spinal glutamate and aspartate concentrations. Glutamate concentrations within the dorsal horn were also increased 1 week after the second acid injection. Our data suggest increased release of spinal EAAs in the dorsal horn contributes to the development and maintenance of hyperalgesia.
D A Skyba; T L Lisi; K A Sluka
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-11-16
Journal Detail:
Title:  Pain     Volume:  119     ISSN:  0304-3959     ISO Abbreviation:  Pain     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7508686     Medline TA:  Pain     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  142-9     Citation Subset:  IM    
Department of Basic Sciences, Palmer College-Florida, Port Orange, FL 32129, USA.
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MeSH Terms
Adaptation, Physiological
Aspartic Acid / metabolism*
Excitatory Amino Acids / metabolism*
Glutamic Acid / metabolism*
Hyperalgesia / chemically induced,  metabolism*
Hyperesthesia / chemically induced,  metabolism*
Injections, Intramuscular / methods
Myofascial Pain Syndromes / chemically induced,  metabolism*
Posterior Horn Cells / metabolism*
Rats, Sprague-Dawley
Sodium Chloride / administration & dosage
Grant Support
Reg. No./Substance:
0/Excitatory Amino Acids; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 7647-14-5/Sodium Chloride

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