Document Detail


Excitatory GABA: How a Correct Observation May Turn Out to be an Experimental Artifact.
MedLine Citation:
PMID:  22529813     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The concept of the excitatory action of GABA during early development is based on data obtained mainly in brain slice recordings. However, in vivo measurements as well as observations made in intact hippocampal preparations indicate that GABA is in fact inhibitory in rodents at early neonatal stages. The apparent excitatory action of GABA seems to stem from cellular injury due to the slicing procedure, which leads to accumulation of intracellular Cl(-) in injured neurons. This procedural artifact was shown to be attenuated through various manipulations such as addition of energy substrates more relevant to the in vivo situation. These observations question the very concept of excitatory GABA in immature neuronal networks.
Authors:
Piotr Bregestovski; Christophe Bernard
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Publication Detail:
Type:  Journal Article     Date:  2012-04-19
Journal Detail:
Title:  Frontiers in pharmacology     Volume:  3     ISSN:  1663-9812     ISO Abbreviation:  Front Pharmacol     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101548923     Medline TA:  Front Pharmacol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  65     Citation Subset:  -    
Affiliation:
INSERM URM 1106, Institut de Neuroscience des Systèmes, Aix-Marseille Université Marseille, France.
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