Document Detail

Excision repair of ultraviolet damage in mammalian cells. Evidence for two steps in the excision of pyrimidine dimers.
MedLine Citation:
PMID:  656544     Owner:  NLM     Status:  MEDLINE    
The incidence of pyrimidine dimer formation and the kinetics of DNA repair in African green monkey kidney CV-1 cells after ultraviolet (UV) irradiation were studied by measuring survival, T4 endonuclease V-sensitive sites, the fraction of pyrimidine dimers in acid-insoluble DNA as determined by thin layer chromatography (TLC), and repair replication. CV-1 cells exhibit a survival curve with extrapolation number n = 7.8 and Do = 2.5 J/m2. Pyrimidine dimers were lost from acid-insoluble DNA more slowly than endonuclease-sensitive sites were lost from or new bases were incorporated into high molecular weight DNA during the course of repair. Growth of CV-1 cultures in [3H]thymidine or X-irradiation (2 or 10 krads) 24 h before UV irradiation had no effect on repair replication induced by 25 J/m2 of UV. These results suggest that pyrimidine dimer excision measurements by TLC are probably unaffected by radiation from high levels of incorporated radionuclides. The endonuclease-sensitive site and TLC measurements can be reconciled by the assumption that pyrimidine dimers are excised from high molecular weight DNA in acid-insoluble oligonucleotides that are slowly degraded to acid-soluble fragments.
J I Williams; J E Cleaver
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  22     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-08-28     Completed Date:  1978-08-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  265-79     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Survival
DNA / radiation effects
DNA Repair*
DNA Replication
Pyrimidine Dimers / metabolism*
Ultraviolet Rays*
Reg. No./Substance:
0/Pyrimidine Dimers; 9007-49-2/DNA; EC 3.1.-/Endonucleases

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