| Excision repair of ultraviolet damage in mammalian cells. Evidence for two steps in the excision of pyrimidine dimers. | |
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MedLine Citation:
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PMID: 656544 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The incidence of pyrimidine dimer formation and the kinetics of DNA repair in African green monkey kidney CV-1 cells after ultraviolet (UV) irradiation were studied by measuring survival, T4 endonuclease V-sensitive sites, the fraction of pyrimidine dimers in acid-insoluble DNA as determined by thin layer chromatography (TLC), and repair replication. CV-1 cells exhibit a survival curve with extrapolation number n = 7.8 and Do = 2.5 J/m2. Pyrimidine dimers were lost from acid-insoluble DNA more slowly than endonuclease-sensitive sites were lost from or new bases were incorporated into high molecular weight DNA during the course of repair. Growth of CV-1 cultures in [3H]thymidine or X-irradiation (2 or 10 krads) 24 h before UV irradiation had no effect on repair replication induced by 25 J/m2 of UV. These results suggest that pyrimidine dimer excision measurements by TLC are probably unaffected by radiation from high levels of incorporated radionuclides. The endonuclease-sensitive site and TLC measurements can be reconciled by the assumption that pyrimidine dimers are excised from high molecular weight DNA in acid-insoluble oligonucleotides that are slowly degraded to acid-soluble fragments. |
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Authors:
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J I Williams; J E Cleaver |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 22 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-08-28 Completed Date: 1978-08-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 265-79 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Survival DNA / radiation effects DNA Repair* DNA Replication Endonucleases Kinetics Mathematics Pyrimidine Dimers / metabolism* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Pyrimidine Dimers; 9007-49-2/DNA; EC 3.1.-/Endonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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