| Excision and transposition activity of Tc1/mariner superfamily transposons in sea urchin embryos. | |
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MedLine Citation:
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PMID: 20192694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tc1/mariner superfamily transposons are used as transformation vectors in various model organisms. The utility of this transposon family is evidenced by the fact that Tc1/mariner transposons have loose host specificity. However, the activity of these transposons has been observed in only a few organisms, and a recent study in the ascidian Ciona intestinalis suggests that not all Tc1/ mariner transposons show loose host specificity. To understand host specificity, we used sea urchins, since they have a long history as materials of embryology and developmental biology. Transposon techniques have not been reported in this organism, despite the likelihood that these techniques would open up many experimental possibilities. Here we tested the activity of three Tc1/ mariner transposons (Minos, Sleeping Beauty, and Frog Prince) in the sea urchin Hemicentrotus pulcherrimus. Minos has both excision and transposition activity in H. pulcherrimus embryos, whereas no excision activity was detected for Sleeping Beauty or Frog Prince. This study suggests that Minos is active in a broad range of non-host organisms and can be used as a transformation tool in sea urchin embryos. |
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Authors:
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Yasunori Sasakura; Junko Yaguchi; Shunsuke Yaguchi; Mamiko Yajima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zoological science Volume: 27 ISSN: 0289-0003 ISO Abbreviation: Zool. Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-02 Completed Date: 2010-04-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8702287 Medline TA: Zoolog Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 256-62 Citation Subset: IM |
Affiliation:
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Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA Transposable Elements / genetics* DNA-Binding Proteins / genetics*, metabolism Hemicentrotus / embryology*, genetics* Transposases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 0/DNA-Binding Proteins; EC 2.7.7.-/Tc1 transposase; EC 2.7.7.-/Transposases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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