Document Detail

Excessive apoptosis of guinea pig colonocytes may lead to an imbalance between phagocytosis and degradation in vivo.
MedLine Citation:
PMID:  14963766     Owner:  NLM     Status:  MEDLINE    
The success or failure of the clearance of apoptotic cell remains depends on the ability of phagocytic cells to recognize, phagocytoze, and digest these remains prior to their lysis, which would cause tissue inflammation. We have recently shown that, after mass-induced apoptosis of guinea pig colonocytes in vivo, phagocytosis by resident macrophages, although efficient, does not prevent a pre-inflammatory response of the mucosa. The present study has investigated the cause(s) of this clearance failure. Immunohistochemistry and transmission electron microscopy were applied. Antibodies directed against the epithelial plasma membrane protein E-cadherin, the lysosomal membrane protein LAMP-1, and the lysosomal matrix protease cathepsin-D were used. The results revealed that: (1) anti-E-cadherin labeled the membrane of epithelial apoptotic bodies internalized in macrophages, (2) double and triple labeling demonstrated that the anti-LAMP-1 and anti-cathepsin-D antibodies recognized and were co-localized in lysosomes and/or phagolysosomes in macrophages but left E-cadherin-positive structures unlabeled, (3) the more numerous were the E-cadherin-positive inclusions in macrophages, the smaller was the number of those that stained positive for lysosomal markers. In parallel with electron microscopy, these findings showed that not all apoptotic bodies phagocytozed by macrophages were subsequently digested, suggesting that the phagocytotic ability of these cells was not matched by their digestive capability.
Stephanie Groos; Roger Busche; Wolfgang von Engelhardt; Enrico Reale; Liliana Luciano
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Publication Detail:
Type:  Journal Article     Date:  2004-02-13
Journal Detail:
Title:  Cell and tissue research     Volume:  316     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-22     Completed Date:  2004-12-15     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  77-86     Citation Subset:  IM    
Department of Microscopic Anatomy, Center of Anatomy, Medical School, Hannover, Germany.
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MeSH Terms
Antigens, CD / metabolism
Apoptosis / physiology*
Cadherins / metabolism
Cathepsin D / metabolism
Colon / physiology*,  ultrastructure
Guinea Pigs
Intestinal Mucosa / physiology*,  ultrastructure
Lysosome-Associated Membrane Glycoproteins
Lysosomes / physiology,  ultrastructure
Macrophages / physiology*,  ultrastructure
Phagocytosis / physiology*
Phagosomes / physiology,  ultrastructure
Reg. No./Substance:
0/Antigens, CD; 0/Cadherins; 0/Lysosome-Associated Membrane Glycoproteins; EC D

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