| Excess expression of uterine ribosomal protein genes P2 and S25 during the "implantation window" in the rat. | |
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MedLine Citation:
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PMID: 9420878 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The present study describes the isolation of "implantation-related" uterine cDNA clones. It further describes their temporal and spatial expression and their identification as the messengers for two ribosomal proteins: RP2 and RS25. METHODS: Libraries of cDNA, representing spayed rats treated with progesterone for 3 consecutive days and with estrogen for either 12 or 36 hours, were screened using homologous and heterologous probes. Two cDNA clones showing differential intensity of the signal were sequenced, the timing of their expression was analyzed by Northern analysis, and their spatial expression was visualized by in situ hybridization. RESULTS: The steady-state level of RP2 mRNA was temporally and spatially controlled by estrogen and progesterone. Whereas estrogen, alone or in combination with progesterone, stimulated this gene, the spatial distribution of the activation was different. Estradiol alone directed RP2 expression to the endometrial-myometrial border, whereas combined treatment increased epithelial staining and directed heavy expression to the stratum vasculare. In normal pregnancy, during the implantation-window period, RP2 was mainly expressed on the mesometrial side, with much less staining on the antimesometrial side. The steady-state levels of the RS25 messenger were elevated by estrogen alone or in combination with progesterone and were confined to the uterine epithelium. RS25 mRNA was evenly distributed throughout the uterine stroma during implantation. CONCLUSION: A developmental pattern of expression of RP2 is reported that corresponds temporally with the primary decidual response but is spatially expressed at the site of the secondary response. |
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Authors:
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T Kidron; P F Kraicer; S Polak-Charcon; A Amit; J B Lessing; U Barkai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of the Society for Gynecologic Investigation Volume: 2 ISSN: 1071-5576 ISO Abbreviation: J. Soc. Gynecol. Investig. Publication Date: 1995 Sep-Oct |
Date Detail:
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Created Date: 1998-01-22 Completed Date: 1998-01-22 Revised Date: 2005-11-05 |
Medline Journal Info:
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Nlm Unique ID: 9433806 Medline TA: J Soc Gynecol Investig Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 700-7 Citation Subset: IM |
Affiliation:
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Department of Zoology, G. S. Wise Faculty of Life Sciences, Ramat Aviv, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary Embryo Implantation / physiology* Endometrium / cytology, metabolism Epithelial Cells / cytology, metabolism Female Gene Library Kinetics Molecular Sequence Data Myometrium / cytology, metabolism Phosphoproteins / biosynthesis* Pregnancy Pregnancy, Animal / metabolism* Protein Biosynthesis RNA, Messenger / biosynthesis Rats Rats, Wistar Ribosomal Proteins / biosynthesis* Transcription, Genetic Uterus / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Phosphoproteins; 0/RNA, Messenger; 0/Ribosomal Proteins; 0/Rps25 protein, rat; 0/phosphoprotein P2, ribosomal |
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