Document Detail

Exceptional transmission of plastids and mitochondria from the transplastomic pollen parent and its impact on transgene containment.
MedLine Citation:
PMID:  17420457     Owner:  NLM     Status:  MEDLINE    
Plastids in Nicotiana tabacum are normally transmitted to the progeny by the maternal parent only. However, low-frequency paternal plastid transmission has been reported in crosses involving parents with an alien cytoplasm. Our objective was to determine whether paternal plastids are transmitted in crosses between parents with the normal cytoplasm. The transplastomic father lines carried a spectinomycin resistance (aadA) transgene incorporated in the plastid genome. The mother lines in the crosses were either (i) alloplasmic, with the Nicotiana undulata cytoplasm that confers cytoplasmic male sterility (CMS92) or (ii) normal, with the fertile N. tabacum cytoplasm. Here we report that plastids from the transplastomic father were transmitted in both cases at low (10(-4)-10(-5)) frequencies; therefore, rare paternal pollen transmission is not simply due to breakdown of normal controls caused by the alien cytoplasm. Furthermore, we have found that the entire plastid genome was transmitted by pollen rather than small plastid genome (ptDNA) fragments. Interestingly, the plants, which inherited paternal plastids, also carried paternal mitochondrial DNA, indicating cotransmission of plastids and mitochondria in the same pollen. The detection of rare paternal plastid transmission described here was facilitated by direct selection for the transplastomic spectinomycin resistance marker in tissue culture; therefore, recovery of rare paternal plastids in the germline is less likely to occur under field conditions.
Zora Svab; Pal Maliga
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-04-09
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-25     Completed Date:  2007-06-06     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7003-8     Citation Subset:  IM    
Waksman Institute, Rutgers-The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA.
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MeSH Terms
Containment of Biohazards*
Crosses, Genetic
Cytoplasm / genetics
DNA Probes
DNA, Chloroplast / genetics
DNA, Mitochondrial / genetics
Drug Resistance
Inheritance Patterns / genetics*
Mitochondria / genetics*
Plant Infertility
Plastids / genetics*
Pollen / genetics*
Polymorphism, Restriction Fragment Length
Selection, Genetic
Tobacco / genetics*
Reg. No./Substance:
0/DNA Probes; 0/DNA, Chloroplast; 0/DNA, Mitochondrial
Comment In:
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):6879-80   [PMID:  17440039 ]

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