Document Detail


Exceptional Stability of Artemin Neurotrophic Factor Dimers: Effects of Temperature, pH, Buffer and Storage Conditions on Protein Integrity and Activity.
MedLine Citation:
PMID:  21892665     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Artemin (ARTN) is a neurotrophic growth factor of the GDNF ligand family that signals through the specific GFRα-3 coreceptor/cRet tyrosine kinase-mediated signaling cascade. Its expression and signaling action in adults are restricted to nociceptive sensory neurons in the dorsal root ganglia. Consequently, Artemin supports survival and growth of sensory neurons and has been studied as a possible treatment for neuropathic pain. We have developed a robust and sensitive cellular assay to measure ARTN biological activity. Using recombinant Artemin produced in Escherichia coli bacteria together with this specific assay, we demonstrate that ARTN is an exceptionally stable polypeptide. Multiple freeze-thaw cycles, incubation at elevated temperatures (up to 90 °C) for 0.5 h, prolonged storage at 4 °C, and exposure to conditions of different pH, salt concentration, and additives had no measurable effect on the biological activity of ARTN. In some of the tested conditions, partial removal of nine NH(2)-terminal amino acids of the ARTN protein occurred, but this truncation had no important effect on the ARTN signaling response. Consequently, we postulate that formulation and storage for in vivo testing of ARTN in neuropathic pain paradigms in animals and humans should be straightforward.
Authors:
Wouter Bruinzeel; Stefan Masure
Related Documents :
22118415 - Activity-dependent survival of developing neocortical neurons depends on pi3k signalling.
19710465 - Mekk1 binds hect e3 ligase itch by its amino-terminal ring motif to regulate th2 cytoki...
20017195 - Energy addiction and lymphocyte differentiation: a new role for the liver kinase b1 kin...
2481585 - The lymphocyte-specific protein tyrosine kinase p56lck is hyperphosphorylated on serine...
10497255 - Phosphorylation of insulin receptor substrate-1 (irs-1) by protein kinase b positively ...
10373445 - Mechanotransduction in response to shear stress. roles of receptor tyrosine kinases, in...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-3
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Assay Development & Target Validation, C.R.E.A.Te, Janssen Research & Development, Turnhoutseweg 30, 2340, Beerse, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of biomass adaptation to biodegradation of dissolved organic carbon in water.
Next Document:  Direct and Indirect Organogenesis of Alpinia galanga and the Phytochemical Analysis.