Document Detail


Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines.
MedLine Citation:
PMID:  19008405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect dicistrovirus with 5' IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 (Spodoptera frugiperda) and BmN (Bombyx mori), and the dipteran cell lines S2 (Drosophila melanogaster) and ATC-10 (Aedes aegypti). Evaluation of dual luciferase expression from DNA plasmids and in vitro-transcribed RNA revealed apparent splicing with certain IRES elements. Though IRES activity depended upon the cell line examined, the black queen cell and Drosophila C dicistrovirus intergenic IRES elements were most effective for coupled gene expression in the diverse insect cell lines examined.
Authors:
James R Carter; Tresa S Fraser; Malcolm J Fraser
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of general virology     Volume:  89     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-14     Completed Date:  2009-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3150-5     Citation Subset:  IM    
Affiliation:
214 Galvin Life Sciences, Department of Biological Sciences, Center for Tropical Diseases Research and Training, University of Notre Dame, PO Box 369, Notre Dame, IN 46556-0369, USA.
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MeSH Terms
Descriptor/Qualifier:
Aedes / virology
Animals
Bombyx / virology
Cell Line
DNA, Intergenic*
Drosophila melanogaster / virology
Enhancer Elements, Genetic*
Humans
Insect Viruses / genetics,  metabolism,  pathogenicity*
Luciferases / genetics,  metabolism
Plasmids / genetics
RNA Splicing
RNA Viruses / genetics,  metabolism,  pathogenicity*
RNA, Messenger / genetics,  metabolism*
RNA, Viral / genetics,  metabolism
Ribosomes / genetics,  metabolism
Spodoptera / virology
Chemical
Reg. No./Substance:
0/DNA, Intergenic; 0/RNA, Messenger; 0/RNA, Viral; EC 1.13.12.-/Luciferases

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