| Examining the relative activity of several dicistrovirus intergenic internal ribosome entry site elements in uninfected insect and mammalian cell lines. | |
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MedLine Citation:
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PMID: 19008405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Comparisons of the relative activities of 11 intergenic region (IGR) internal ribosome entry site (IRES) elements of insect dicistrovirus with 5' IRES elements of the hepatitis C and encephalomyocarditis viruses were performed in insect and mammalian cells. Dual luciferase assays were performed to determine the most effective dicistrovirus IGR IRES in the lepidopteran cell lines Sf9 (Spodoptera frugiperda) and BmN (Bombyx mori), and the dipteran cell lines S2 (Drosophila melanogaster) and ATC-10 (Aedes aegypti). Evaluation of dual luciferase expression from DNA plasmids and in vitro-transcribed RNA revealed apparent splicing with certain IRES elements. Though IRES activity depended upon the cell line examined, the black queen cell and Drosophila C dicistrovirus intergenic IRES elements were most effective for coupled gene expression in the diverse insect cell lines examined. |
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Authors:
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James R Carter; Tresa S Fraser; Malcolm J Fraser |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of general virology Volume: 89 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-14 Completed Date: 2009-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 3150-5 Citation Subset: IM |
Affiliation:
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214 Galvin Life Sciences, Department of Biological Sciences, Center for Tropical Diseases Research and Training, University of Notre Dame, PO Box 369, Notre Dame, IN 46556-0369, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aedes
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virology Animals Bombyx / virology Cell Line DNA, Intergenic* Drosophila melanogaster / virology Enhancer Elements, Genetic* Humans Insect Viruses / genetics, metabolism, pathogenicity* Luciferases / genetics, metabolism Plasmids / genetics RNA Splicing RNA Viruses / genetics, metabolism, pathogenicity* RNA, Messenger / genetics, metabolism* RNA, Viral / genetics, metabolism Ribosomes / genetics, metabolism Spodoptera / virology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Intergenic; 0/RNA, Messenger; 0/RNA, Viral; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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