Document Detail


Examining the role of mechanosensitive ion channels in pressure mechanotransduction in rat bladder urothelial cells.
MedLine Citation:
PMID:  21104316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Until recently, the bladder urothelium had been thought of only as a physical barrier between urine and underlying bladder tissue. Recent studies, however, have demonstrated that the urothelium is sensitive to mechanical stimuli and responds by releasing signaling molecules (NO, ATP). This study sought to investigate the role of select ion channels in urothelial cell (UC) pressure mechanotransduction. Using a custom-made pressure chamber, rat bladder UCs cultured on tissue culture plastic dishes were exposed to sustained hydrostatic pressure (5-20 cmH(2)O) for up to 30 min. When compared to the control, UCs exposed to 10 cmH(2)O (5 min), and 15 cmH(2)O (5 and 15 min), exhibited a significant (p < 0.05) increase in ATP release. In the absence of extracellular calcium, ATP release due to hydrostatic pressure was attenuated. Blocking the L-type voltage-gated channel with nifedipine during pressure exposure did not affect ATP release. However, blocking TRP channels, stretch-activated channels (SACs), and the epithelial sodium channel (ENaC) with ruthenium red, gadolinium chloride, and amiloride, respectively, all abolished hydrostatic pressure-evoked ATP release. These results have provided evidence for the first time that cultured UCs are sensitive to hydrostatic pressure in the physiologically relevant range. The results of this study also provide evidence that one or multiple mechanosensitive ion channels play a role in the mechanotransduction of hydrostatic pressure, which supports the view that not only tissue stretch or tension, but also pressure is an important parameter for mechanosensing of bladder fullness.
Authors:
Shawn M Olsen; Joshua D Stover; Jiro Nagatomi
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Publication Detail:
Type:  Journal Article     Date:  2010-11-23
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  39     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-04     Completed Date:  2011-05-27     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  688-97     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, 301 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634-0905, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism*
Animals
Female
Ion Channel Gating / physiology*
Ion Channels / physiology*
Mechanotransduction, Cellular / physiology*
Pressure
Rats
Rats, Sprague-Dawley
Urinary Bladder / cytology,  physiology*
Urothelium / cytology,  physiology*
Chemical
Reg. No./Substance:
0/Ion Channels; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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