Document Detail


Examination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype 1 grown under iron-restricted conditions.
MedLine Citation:
PMID:  8998986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the expression of important Actinobacillus pleuropneumoniae surface polysaccharides, namely, capsular polysaccharides (CPS) and lipopolysaccharides (LPS), after growth under iron-restricted conditions. Iron restriction did not seem to affect the production of CPS, as determined by labelling with a monoclonal antibody (mAb) against the serotype 1 K-antigen and flow cytometry analysis, and also as determined by electron microscopy. SDS-PAGE revealed that the LPS profiles of these cells were also unaffected by iron restriction. Using flow cytometry analysis, however, we observed that binding of mAb against serotype 1 O-antigen was altered in cells of A. pleuropneumoniae serotype 1 reference strain (4074) grown under iron-restricted conditions. This strain exhibited two subpopulations with distinct patterns of reactivity with the mAb against the O-antigen. When strain 4074 was grown under iron-restricted conditions, a shift from one cell subpopulation (moderately fluorescent) to another cell subpopulation (highly fluorescent, thus binding more antibodies) was observed. Our results indicate that growth of A. pleuropneumoniae serotype 1 under iron-restricted conditions did not seem to affect CPS production, but might alter, at least for the reference strain, the expression of LPS.
Authors:
S E Paradis; D Dubreuil; M Jacques
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  137     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-01-17     Completed Date:  1997-01-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  201-6     Citation Subset:  IM    
Affiliation:
Département de pathologie et microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Actinobacillus pleuropneumoniae / classification,  growth & development,  metabolism*
Antibodies, Monoclonal
Bacterial Capsules / biosynthesis,  immunology
Flow Cytometry
Iron / metabolism*
Lipopolysaccharides / biosynthesis,  immunology
Microscopy, Immunoelectron
O Antigens / biosynthesis
Polysaccharides, Bacterial / biosynthesis*,  immunology
Serotyping
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bacterial Capsules; 0/Lipopolysaccharides; 0/O Antigens; 0/Polysaccharides, Bacterial; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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