Document Detail

Examination of serum and bovine serum albumin as shear protective agents in agitated cultures of hybridoma cells.
MedLine Citation:
PMID:  1367099     Owner:  NLM     Status:  MEDLINE    
A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible to shear in the stationary and in the decline phase of growth as evidenced by higher death rates. Cell growth was best at high serum concentrations with high specific growth and low specific death rates. When BSA was used instead of serum in IMDM, no protective effect was observed. Cell metabolism and monoclonal antibody production rates were not influenced by the level of serum or by BSA. The use of serum in commercial serum-free media (OPTI-MEM) also resulted in no change in both growth and death rates.
S S Ozturk; B O Palsson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biotechnology     Volume:  18     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-06-10     Completed Date:  1991-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  13-28     Citation Subset:  B    
Department of Chemical Engineering, University of Michigan, Ann Arbor 48109.
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MeSH Terms
Antibody Formation
Blood Physiological Phenomena*
Cell Survival
Cells, Cultured
Culture Media
Hybridomas / cytology*,  immunology,  metabolism
Mice, Inbred BALB C
Serum Albumin, Bovine / pharmacology*
Reg. No./Substance:
0/Culture Media; 0/Serum Albumin, Bovine

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