| Examination of a rodent limb bud micromass assay as a prescreen for developmental toxicity. | |
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MedLine Citation:
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PMID: 2326758 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mouse limb bud micromass assay is one of many short-term tests proposed as preliminary screens for potential developmental toxicity. Previous efforts to validate this assay have used too few "nonteratogens." The purpose of this study was to examine additional compounds, most of which, based on the literature, were perceived to have low potential for developmental toxicity in vivo. In addition, a method of data analysis was sought that would identify selective developmental toxins in the micromass assay, i.e., those that are effective at dosages not maternally toxic. The concentration of each of 23 compounds that produced a 50% inhibition (IC50) of radiolabeled thymidine (T) and sulfate (S) incorporation was determined and used to calculate a T/S ratio. The T/S ratio may be a useful measure of developmental hazard, since T incorporation measures toxicity toward a general cell function (DNA synthesis) and S incorporation measures mainly toxicity toward a developmentally specific cell activity (chondroitin sulfate synthesis). All compounds tested produced T/S ratios of less than 2.0. Since 22 of these 23 compounds are classified as "nonteratogens" or nonselective developmental toxins in vivo, a low T/S ratio in this in vitro assay system may be capable of discriminating potential for developmental hazard in vivo. |
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Authors:
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L D Wise; R L Clark; J O Rundell; R T Robertson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Teratology Volume: 41 ISSN: 0040-3709 ISO Abbreviation: Teratology Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-05-21 Completed Date: 1990-05-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0153257 Medline TA: Teratology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 341-51 Citation Subset: IM |
Affiliation:
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Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Drug Evaluation, Preclinical / methods Female Limb Deformities, Congenital* Mice Pregnancy Teratogens* Toxicology / methods* |
| Chemical | |
Reg. No./Substance:
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0/Teratogens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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