Document Detail

Examination of meat components in commercial dog and cat feed by using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique.
MedLine Citation:
PMID:  15297759     Owner:  NLM     Status:  MEDLINE    
It has been shown that certain slow neurological diseases such as bovine spongiform encephalopathy (also known as "mad cow" disease) could be transmitted through contaminated food intake by animals; therefore, the examination of meat components in commercial feeds is important for the control of the disease in public health. The combination of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique was applied to examine the meat components in dog and cat commercial feeds. The partial nucleotide sequence (359 bp) of animal mitochondrial cytochrome b (cytb, CYT) gene was amplified by PCR and then digested with restriction enzyme Alu I or Mbo I. In this work, eight brands of commercial dog and cat feeds available in Taiwan were examined. All brands of dog feeds that were tested contained meat from four different animals (cattle, pig, goat and chicken). In cat feeds, the chicken meat was found in five out of eight brands.
Hsien-Chi Wang; Shu-Hwae Lee; Tien-Jye Chang; Min-Liang Wong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  66     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-08-06     Completed Date:  2004-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  855-9     Citation Subset:  IM    
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
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MeSH Terms
Animal Feed / analysis*
Cytochromes b / genetics
DNA / isolation & purification
DNA Restriction Enzymes
Electrophoresis, Agar Gel
Meat Products / analysis*
Mitochondria / enzymology
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Reg. No./Substance:
9007-49-2/DNA; 9035-37-4/Cytochromes b; EC 3.1.21.-/DNA Restriction Enzymes

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