| Examination of meat components in commercial dog and cat feed by using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique. | |
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MedLine Citation:
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PMID: 15297759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been shown that certain slow neurological diseases such as bovine spongiform encephalopathy (also known as "mad cow" disease) could be transmitted through contaminated food intake by animals; therefore, the examination of meat components in commercial feeds is important for the control of the disease in public health. The combination of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique was applied to examine the meat components in dog and cat commercial feeds. The partial nucleotide sequence (359 bp) of animal mitochondrial cytochrome b (cytb, CYT) gene was amplified by PCR and then digested with restriction enzyme Alu I or Mbo I. In this work, eight brands of commercial dog and cat feeds available in Taiwan were examined. All brands of dog feeds that were tested contained meat from four different animals (cattle, pig, goat and chicken). In cat feeds, the chicken meat was found in five out of eight brands. |
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Authors:
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Hsien-Chi Wang; Shu-Hwae Lee; Tien-Jye Chang; Min-Liang Wong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of veterinary medical science / the Japanese Society of Veterinary Science Volume: 66 ISSN: 0916-7250 ISO Abbreviation: J. Vet. Med. Sci. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-08-06 Completed Date: 2004-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9105360 Medline TA: J Vet Med Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 855-9 Citation Subset: IM |
Affiliation:
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Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis* Animals Cats Cattle Chickens Cytochromes b / genetics DNA / isolation & purification DNA Restriction Enzymes Dogs Electrophoresis, Agar Gel Goats Meat Products / analysis* Mitochondria / enzymology Polymerase Chain Reaction / methods Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Swine Taiwan |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA; 9035-37-4/Cytochromes b; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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