Document Detail

Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers.
MedLine Citation:
PMID:  12752646     Owner:  NLM     Status:  MEDLINE    
AIM: To study in vitro the cytotoxic and mutagenic effects of AH26 and AH Plus.
METHODOLOGY: Cytotoxic effects on Chinese hamster V79 cells were determined by counting viable cells following incubation with eluations of AH26 and AH Plus. In one set of experiments, the materials were mixed, set for 1 h and then eluted with dimethyl sulphoxide (DMSO) for 1 h, 24 h and 7 days. In the other set, AH26 and AH Plus were mixed and set for 1 h, 24 h and 7 days in physiological saline then crushed and eluted in DMSO for 24 h. The cytotoxic effects of these eluates were evaluated. Three concentrations were chosen to examine the mutagenic effects of AH26 and AH Plus: 5.57, 16.7 and 55.7 microg mL(-1). The structural chromosomal aberration analysis and micronucleus test were performed on human lymphocytes according to standard procedures.
RESULTS: Dose-response curves of cell survival were obtained. Both materials were shown to be cytotoxic in doses larger than 55.7 microg mL(-1), except for AH26, after 7 days setting time. AH Plus was also shown to be toxic in concentrations of 16.7 microg mL(-1), except after 7 days setting time. Neither AH26 nor AH Plus induced a significant increase of chromosomal aberrations or micronuclei induction at any setting time or concentration.
CONCLUSION: There was no mutagenicity found for AH26 and AH Plus on human lymphocytes in highly controlled conditions in vitro.
I Miletić; S Jukić; I Anić; D Zeljezić; V Garaj-Vrhovac; M Osmak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International endodontic journal     Volume:  36     ISSN:  0143-2885     ISO Abbreviation:  Int Endod J     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-19     Completed Date:  2003-08-05     Revised Date:  2012-03-05    
Medline Journal Info:
Nlm Unique ID:  8004996     Medline TA:  Int Endod J     Country:  England    
Other Details:
Languages:  eng     Pagination:  330-5     Citation Subset:  D    
School of Dentistry, University of Zagreb, Croatia.
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MeSH Terms
Bismuth / administration & dosage,  toxicity*
Cell Line
Cell Survival / drug effects
Chromosome Aberrations / chemically induced
Dimethyl Sulfoxide
Dose-Response Relationship, Drug
Drug Combinations
Epoxy Resins / administration & dosage,  toxicity*
Fibroblasts / drug effects
Lymphocytes / drug effects
Micronuclei, Chromosome-Defective / drug effects
Mutagens / administration & dosage,  toxicity*
Root Canal Filling Materials / toxicity*
Silver / administration & dosage,  toxicity*
Time Factors
Titanium / administration & dosage,  toxicity*
Reg. No./Substance:
0/Drug Combinations; 0/Epoxy Resins; 0/Mutagens; 0/Root Canal Filling Materials; 0/Solvents; 0/expoy resin-based root canal sealer; 55599-25-2/epoxy resin AH-26; 67-68-5/Dimethyl Sulfoxide; 7440-22-4/Silver; 7440-32-6/Titanium; 7440-69-9/Bismuth

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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