Document Detail


Examination of aggregation of various red blood cell populations can be informative in comparison of splenectomy and spleen autotransplantation in animal experiments.
MedLine Citation:
PMID:  20675909     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using "Furka's spleen-chip" autotransplantation technique, in a follow-up study we investigated erythrocyte aggregation and its influencing factors regarding to asplenic-hyposplenic conditions. Healthy beagle dogs were equally divided into 4 groups: "Sham"- sham operated control, "SE"-splenectomy, "AU-5" and "AU-10"-autotransplantation with 5 or 10 spleen chips by Furka-method after splenectomy. Blood sampling for current investigation occurred on the 12th, 18th, 20th and 24th postoperative months, besides comprehensive structural and functional examinations. In this study we focused on the aggregation properties of erythrocytes in native samples and of density separated "young" versus "old" erythrocytes (Myrenne MA-1 aggregometer). Fibrinogen concentration (Sysmex CA-500 coagulometer) and erythrocyte sedimentation rate (ESR) were also determined. SE group showed significantly elevated ratio of "old" and "young" erythrocyte aggregation index values on the 20th and 24th postoperative months (1.5 and 2.16, respectively) comparing to the AU (1.14 and 1.05) and Sham groups (1.2 and 1.27). In SE group fibrinogen concentration was mildly higher and ESR almost doubled versus control. The greater differences experienced between "young" and "old" erythrocytes may reflect decreased splenic filtration function. Consequently, the comparison of aggregation and deformability properties of "young" and "old" erythrocytes may give important information about the function of the spleen.
Authors:
Ferenc Kiss; Norbert Nemeth; Erika Sajtos; Endre Brath; Katalin Peto; Oguz K Baskurt; Istvan Furka; Iren Miko
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical hemorheology and microcirculation     Volume:  45     ISSN:  1875-8622     ISO Abbreviation:  Clin. Hemorheol. Microcirc.     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-02     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9709206     Medline TA:  Clin Hemorheol Microcirc     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
Affiliation:
Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary. kife@med.unideb.hu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Sedimentation
Dogs
Erythrocyte Aggregation*
Erythrocyte Aging*
Erythrocyte Deformability
Fibrinogen / analysis
Follow-Up Studies
Spleen / transplantation*
Splenectomy*
Transplantation, Autologous
Chemical
Reg. No./Substance:
9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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