Document Detail


Ex vivo stability of the rodent-borne Hantaan virus in comparison to that of arthropod-borne members of the Bunyaviridae family.
MedLine Citation:
PMID:  17337567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. In this study we have compared the stabilities of three viruses within the Bunyaviridae family: the rodent-borne Hantavirus Hantaan virus (HTNV), the sand fly-borne Phlebovirus sandfly fever Sicilian virus (SFSV), and the tick-borne Nairovirus Crimean-Congo hemorrhagic fever virus (CCHFV). These viruses differ in their transmission routes: SFSV and CCHFV are vector borne, whereas HTNV is spread directly between its hosts, and to humans, via the environment. We studied whether these viruses differed regarding stability when kept outside of the host. Viral survival was analyzed at different time points upon exposure to different temperatures (4 degrees C, 20 degrees C, and 37 degrees C) and drying at 20 degrees C. We observed clearly different stabilities under wet conditions, particularly at 4 degrees C, where infectious SFSV, HTNV, and CCHFV were detectable after 528, 96, and 15 days, respectively. All three viruses were equally sensitive to drying, as shown by drying on aluminum discs. Furthermore, HTNV and SFSV partially survived for 2 min in 30% ethanol, whereas CCHFV did not. Electron microscopy images of HTNV, SSFSV, and CCHFV stored at 37 degrees C until infectivity was lost still showed the occurrence of virions, but with abnormal shapes and densities compared to those of the nonincubated samples. In conclusion, our study points out important differences in ex vivo stability among viruses within the Bunyaviridae family.
Authors:
J Hardestam; M Simon; K O Hedlund; A Vaheri; J Klingström; A Lundkvist
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-02
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  73     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-12     Completed Date:  2007-10-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2547-51     Citation Subset:  IM    
Affiliation:
Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden. jonas.hardestam@smi.ki.se
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MeSH Terms
Descriptor/Qualifier:
Antiviral Agents / pharmacology
Ethanol / pharmacology
Hantaan virus / drug effects,  physiology*,  ultrastructure
Hemorrhagic Fever Virus, Crimean-Congo / drug effects,  physiology*,  ultrastructure
Microbial Viability*
Microscopy, Electron, Transmission
Phlebovirus / drug effects,  physiology*,  ultrastructure
Temperature
Time Factors
Virion / ultrastructure
Chemical
Reg. No./Substance:
0/Antiviral Agents; 64-17-5/Ethanol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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