| Ex vivo imaging of excised tissue using vital dyes and confocal microscopy. | |
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MedLine Citation:
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PMID: 22752953 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here are useful for structural and functional analysis of viable properties of cells in intact tissue slices, allowing for the collection of data in a structurally relevant environment. With these protocols, vital dyes can be applied as a research tool to disease processes and properties of tissue not amenable to cell culture-based studies. |
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Authors:
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Simon Johnson; Peter Rabinovitch |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 9 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-07-03 Completed Date: 2012-10-29 Revised Date: 2013-04-22 |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit 9.39 Citation Subset: IM |
Affiliation:
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University of Washington, Seattle, Washington, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / metabolism Cell Survival Coloring Agents / analysis* Data Collection Ethidium / analogs & derivatives, metabolism Fluoresceins / metabolism Green Fluorescent Proteins / metabolism Imaging, Three-Dimensional / methods* Mice Microscopy, Confocal / methods* Mitochondria / metabolism Organ Specificity* Organic Chemicals / metabolism Organometallic Compounds / metabolism Staining and Labeling / methods* Tissue Survival |
| Grant Support | |
ID/Acronym/Agency:
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P01 AG001751/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; T32 AG000057/AG/NIA NIH HHS; T32 AG000057/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 0/Fluoresceins; 0/Organic Chemicals; 0/Organometallic Compounds; 0/SYTOX Green; 0/tetramethyl rhodamine ethyl ester; 147336-22-9/Green Fluorescent Proteins; 148504-34-1/calcein AM; 3546-21-2/Ethidium; 61926-22-5/ethidium homodimer |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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