Document Detail


Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.
MedLine Citation:
PMID:  22752953     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here are useful for structural and functional analysis of viable properties of cells in intact tissue slices, allowing for the collection of data in a structurally relevant environment. With these protocols, vital dyes can be applied as a research tool to disease processes and properties of tissue not amenable to cell culture-based studies.
Authors:
Simon Johnson; Peter Rabinovitch
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 9     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-10-29     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 9.39     Citation Subset:  IM    
Affiliation:
University of Washington, Seattle, Washington, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / metabolism
Cell Survival
Coloring Agents / analysis*
Data Collection
Ethidium / analogs & derivatives,  metabolism
Fluoresceins / metabolism
Green Fluorescent Proteins / metabolism
Imaging, Three-Dimensional / methods*
Mice
Microscopy, Confocal / methods*
Mitochondria / metabolism
Organ Specificity*
Organic Chemicals / metabolism
Organometallic Compounds / metabolism
Staining and Labeling / methods*
Tissue Survival
Grant Support
ID/Acronym/Agency:
P01 AG001751/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; T32 AG000057/AG/NIA NIH HHS; T32 AG000057/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Fluoresceins; 0/Organic Chemicals; 0/Organometallic Compounds; 0/SYTOX Green; 0/tetramethyl rhodamine ethyl ester; 147336-22-9/Green Fluorescent Proteins; 148504-34-1/calcein AM; 3546-21-2/Ethidium; 61926-22-5/ethidium homodimer
Comments/Corrections

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