Document Detail

Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.
MedLine Citation:
PMID:  22752953     Owner:  NLM     Status:  MEDLINE    
Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here are useful for structural and functional analysis of viable properties of cells in intact tissue slices, allowing for the collection of data in a structurally relevant environment. With these protocols, vital dyes can be applied as a research tool to disease processes and properties of tissue not amenable to cell culture-based studies.
Simon Johnson; Peter Rabinovitch
Related Documents :
24865213 - Evaluation of automatic exposure control options in digital mammography.
19129923 - Slm microscopy: scanless two-photon imaging and photostimulation with spatial light mod...
11203933 - Effects of different exposure values on diagnostic accuracy of digital images.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 9     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-03     Completed Date:  2012-10-29     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 9.39     Citation Subset:  IM    
University of Washington, Seattle, Washington, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Nucleus / metabolism
Cell Survival
Coloring Agents / analysis*
Data Collection
Ethidium / analogs & derivatives,  metabolism
Fluoresceins / metabolism
Green Fluorescent Proteins / metabolism
Imaging, Three-Dimensional / methods*
Microscopy, Confocal / methods*
Mitochondria / metabolism
Organ Specificity*
Organic Chemicals / metabolism
Organometallic Compounds / metabolism
Staining and Labeling / methods*
Tissue Survival
Grant Support
P01 AG001751/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; P30 AG013280/AG/NIA NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; R01 HL101186/HL/NHLBI NIH HHS; T32 AG000057/AG/NIA NIH HHS; T32 AG000057/AG/NIA NIH HHS
Reg. No./Substance:
0/Coloring Agents; 0/Fluoresceins; 0/Organic Chemicals; 0/Organometallic Compounds; 0/SYTOX Green; 0/tetramethyl rhodamine ethyl ester; 147336-22-9/Green Fluorescent Proteins; 148504-34-1/calcein AM; 3546-21-2/Ethidium; 61926-22-5/ethidium homodimer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues.
Next Document:  Circulating CXCL9 and CXCL10 as Markers of Activity of Graves' Orbitopathy During Treatment with Cor...