Document Detail


Ex vivo expansion of haematopoietic stem cells to improve engraftment in stem cell transplantation.
MedLine Citation:
PMID:  21755454     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The efficient use of haematopoietic stem cells (HSC) for transplantation is often limited by the relatively low numbers of HSC collected. The ex vivo expansion of HSC for clinical use is a potentially valuable and safe approach to increase HSC numbers thereby increasing engraftment and reducing the risk of morbidity from infection. Here we describe a protocol for the robust ex vivo expansion of human CD34(+) HSC isolated from umbilical cord blood. The protocol described can efficiently generate large numbers of HSC. We also describe a flow cytometry-based method using high resolution division tracking to characterise the kinetics of HSC growth and differentiation. Utilising the guidelines discussed, it is possible for investigators to use this protocol as presented or to modify it for their specific needs.
Authors:
Kap-Hyoun Ko; Robert Nordon; Tracey A O'Brien; Geoff Symonds; Alla Dolnikov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  761     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-07-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-60     Citation Subset:  IM    
Affiliation:
Sydney Cord & Marrow Transplant Facility, Graduate School of Biomedical Engineering, Sydney Children's Hospital, University of New South Wales, Kensington, NSW, Australia, kap-hyounk@miltenyibiotec.com.au.
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