| Ex-vivo evaluation of alginate microparticles for Polymyxin B oral administration. | |
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MedLine Citation:
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PMID: 17090395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A crosslinked alginate microparticle system for the targeting to the lymphatic system by Peyer's patches (PP) uptake was designed in order to improve the oral absorption of Polymyxin B (PMB). To verify mucoadhesion and PP uptake, microparticles labelled with fluorescein isothiocyanate (FITC) were prepared by spray-drying technique and crosslinking reactions with calcium ions and chitosan (CS), in vitro characterized and assayed by an ex vivo method. Microparticles showed a size less then 3 microm, an antibiotic loading level of 11.86 +/- 0.70%, w/w, a sustained drug release behaviour in simulated gastro-intestinal (GI) fluids and a preserved biological activity throughout the manufacture. The ex vivo study was performed by a perfusion method on intestinal tracts of just sacrificed adult rats. The recovered samples were analysed by epifluorescence microscope for mucoadhesion and PP uptake and by microbiological analysis for antibiotic activity preservation, providing evidence of mucoadhesion at the level of both PP and non-PP epithelium, uptake by PP and PMB microbiological activity in PP tissue. Furthermore, the study revealed the involvement of transport pathways across villous enterocytes. |
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Authors:
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Gilberto Coppi; Nicoletta Sala; Moreno Bondi; Santo Sergi; Valentina Iannuccelli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of drug targeting Volume: 14 ISSN: 1061-186X ISO Abbreviation: J Drug Target Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-08 Completed Date: 2007-02-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9312476 Medline TA: J Drug Target Country: England |
Other Details:
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Languages: eng Pagination: 599-606 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via Campi 183, 41100, Modena, Italy. coppi.gilberto@unimore.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Alginates / pharmacology* Animals Anti-Bacterial Agents / administration & dosage*, analysis, pharmacokinetics Excipients Fluorescein-5-isothiocyanate Fluorescent Dyes Intestine, Small / drug effects, metabolism Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Nanoparticles Particle Size Perfusion Polymyxin B / administration & dosage*, analysis, pharmacokinetics Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Anti-Bacterial Agents; 0/Excipients; 0/Fluorescent Dyes; 1404-26-8/Polymyxin B; 3326-32-7/Fluorescein-5-isothiocyanate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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