Document Detail

Ex vivo characterization of human atherosclerotic iliac plaque components using cryo-imaging.
MedLine Citation:
PMID:  19094020     Owner:  NLM     Status:  MEDLINE    
We characterized atherosclerotic plaque components with a novel cryo-imaging system in lieu of standard histological methods commonly used for imaging validation and research endpoints. We aim to accurately identify plaque tissue types from fresh cadaver specimens rapidly (less than 5 h) in three dimensions for large specimens (up to 4 cm vessel segments). A single-blind validation study was designed to determine sensitivity, specificity and inter-rater agreement (Fleiss' Kappa) of cryo-imaging tissue types with histology as the gold standard. Six naïve human raters identified 344 tissue type samples in 36 cryo-image sets after being trained. Tissue type sensitivities are as follows: greater than 90% for adventitia, media-related, smooth muscle cell ingrowth, external elastic lamina, internal elastic lamina, fibrosis, dense calcification and haemorrhage; greater than 80% for lipid and light calcification; and greater than 50% for cholesterol clefts. Specificities were greater than 95% for all tissue types. The results demonstrate convincingly that cryo-imaging can be used to accurately identify most tissue types. If the cryo-imaging data are entered into visualization software, three-dimensional renderings of the plaque can be generated to visualize and quantify plaque components.
M S Nguyen; O Salvado; D Roy; G Steyer; M E Stone; R D Hoffman; D L Wilson
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Validation Studies    
Journal Detail:
Title:  Journal of microscopy     Volume:  232     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-19     Completed Date:  2009-01-16     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  432-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aged, 80 and over
Atherosclerosis / pathology*
Histological Techniques / methods*
Iliac Artery / pathology*
Imaging, Three-Dimensional / methods*
Middle Aged
Sensitivity and Specificity
Grant Support
R41 CA124270/CA/NCI NIH HHS; R41 CA124270-01A1/CA/NCI NIH HHS; R41 HL084822/HL/NHLBI NIH HHS; R41 HL084822-01/HL/NHLBI NIH HHS; R41HL084822/HL/NHLBI NIH HHS; R42 CA124270/CA/NCI NIH HHS; R42 CA124270-02/CA/NCI NIH HHS; T35 HL082544/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Use of atomic force microscopy (AFM) for microfabric study of cohesive soils.
Next Document:  Morphology of the fertilizable mature egg in the Acanthopagrus latus, A. schlegeli and Sparus sarba ...