| Ex vivo assessment of mouse cervical remodeling through pregnancy via 23Na MRS. | |
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MedLine Citation:
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PMID: 20878968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Preterm birth occurs in 12.5% of births in the United States and can lead to risk of infant death or to lifelong serious health complications. A greater understanding by which the two main processes, uterine contraction and cervical remodeling are regulated is required to reduce rates of preterm birth. The cervix must undergo extensive remodeling through pregnancy in preparation for parturition, the process of labor and delivery of young. One key aspect of this dynamic process is a change in the composition and abundance of glycosaminoglycans (GAGs) and proteoglycans within the extracellular matrix, which influences the loss of tensile strength or stiffness of the cervix during labor. 23Na NMR spectroscopy has previously been validated as a method to quantify GAGs in tissues. In the current study, the Na+ concentration was measured at several time points through pregnancy in mouse cervices using 23Na NMR spectroscopy. The Na+ concentration increased progressively during pregnancy and peaked one day before birth followed by a rapid decline after birth. The same trend was seen in GAGs as measured by a biochemical assay using independent cervix samples over the course of pregnancy. We suggest that monitoring the Na+ concentration via 23Na NMR spectroscopy can serve as an informative physiological marker in evaluating the stages of cervical remodeling ex vivo and warrants further investigation to determine its utility as a diagnostic tool for the identification of women at risk for impending preterm birth. |
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Authors:
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Xiang Xu; Yucel Akgul; Mala Mahendroo; Alexej Jerschow |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: NMR in biomedicine Volume: 23 ISSN: 1099-1492 ISO Abbreviation: NMR Biomed Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-29 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: England |
Other Details:
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Languages: eng Pagination: 907-12 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 John Wiley & Sons, Ltd. |
Affiliation:
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Chemistry Department, New York University, New York 10003, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis* Cervix Uteri* / anatomy & histology, chemistry, physiology Extracellular Matrix / chemistry Female Gestational Age Glycosaminoglycans / analysis* Mice Mice, Inbred C57BL Parturition / physiology Pregnancy Pregnancy, Animal* Premature Birth / prevention & control Proteoglycans / analysis Sodium Isotopes / metabolism* Water / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1R21AR054002-01A1/AR/NIAMS NIH HHS; P01 HD11149/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Glycosaminoglycans; 0/Proteoglycans; 0/Sodium Isotopes; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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