| Ex vivo activation and expansion of natural killer cells from patients with advanced cancer with feeder cells from healthy volunteers. | |
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MedLine Citation:
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PMID: 23321334 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND AIMS: Culturing natural killer (NK) cells from patients with advanced cancer is difficult and has restricted the generation of sufficient cell numbers for autologous adoptive NK-cell therapy. The aim of this study was to establish a novel method for ex vivo NK-cell expansion from patients with cancer. METHODS: NK cells (CD3(-)CD56(+)) were isolated from peripheral blood mononuclear cells from healthy volunteers and cancer patients, and NK(-) fractions were used as feeder cells. Purified NK cells were co-cultured with feeder cells in AIM-V medium (Invitrogen, Carlsbad, CA, USA) supplemented with 5% human serum and 1000 units/mL human interleukin-2. RESULTS: NK cells co-cultured with feeder cells from healthy volunteers (feeder-HV) expanded more than NK cells co-cultured with feeder cells from cancer patients (feeder-CP). During the 14-day culture period, NK cells from patients with advanced cancer co-cultivated with feeder-HV expanded on average 300-fold. NK cells co-cultivated with feeder-CP expanded on average 169.4-fold. Cultures grown in the presence of feeder-HV contained 93.8 ± 7.0% (mean ± standard deviation; n = 6) CD3(-)CD56(+) NK cells, and cultures grown in the presence of feeder-CP contained 83.6 ± 15.9% CD3(-)CD56(+) NK cells. Feeder-HV caused a relative increase in CD3(+)CD4(+) T cells, whereas feeder-CP did not induce changes. Interleukin-15, a cytokine that induces NK-cell proliferation, was detected in the culture supernatants of feeder-HV but not in those of feeder-CP. CONCLUSIONS: Feeder cells obtained from healthy volunteers have the potential to expand and activate NK cells from patients with advanced cancer. The novel NK-cell expansion method described here provides a technique for acquiring the large numbers of highly active NK cells from patients with cancer for autologous adoptive immunotherapy. |
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Authors:
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Eun-Kyung Kim; Yong-Oon Ahn; Saerom Kim; Tae Min Kim; Bhumsuk Keam; Dae Seog Heo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotherapy Volume: 15 ISSN: 1477-2566 ISO Abbreviation: Cytotherapy Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100895309 Medline TA: Cytotherapy Country: England |
Other Details:
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Languages: eng Pagination: 231-241.e1 Citation Subset: IM |
Copyright Information:
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Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Cancer Research Institute, Seoul National University College of Medicine and Hospital, Seoul, Korea; Innovative Research Institute for Cell Therapy, Seoul National University College of Medicine and Hospital, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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