| Evolutionarily distinct classes of S27 ribosomal proteins with differential mRNA expression in rat hypothalamus. | |
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MedLine Citation:
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PMID: 10820185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using an in situ hybridization screen for cDNA clones of brain region-specific mRNAs, we isolated a rat transcript that encodes a ribosomal protein S27. Searching GenBank DNA databases, we found two S27 protein isoforms. One isoform, encoded by multiple genes, is extant in archaea and eukarya, but not bacteria. The second isoform appears to be recently evolved because it has been identified only in mammals. Multiple transcripts encode each isoform and exhibit different tissue expression patterns throughout rat brain and periphery, with abundant expression in the hypothalamus. In situ hybridization studies revealed predominant expression of S27(1) in distinct hypothalamic nuclei, such as the paraventricular, supraoptic, suprachiasmatic, arcuate, and circularis nuclei, whereas expression of S27(2) mRNA was discretely expressed in select neurons of the periventricular and supraoptic nuclei. Combined with the genetic evidence that S27 has extraribosomal functions in plants, the complexity of S27 biology observed here may suggest auxiliary functions for S27 proteins in the mammalian nervous system. |
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Authors:
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E A Thomas; C E Alvarez; J G Sutcliffe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 74 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 2000 Jun |
Date Detail:
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Created Date: 2000-06-02 Completed Date: 2000-06-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2259-67 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cloning, Molecular DNA, Complementary Evolution, Molecular* Hypothalamus / chemistry, physiology* In Situ Hybridization Isomerism Male Metalloproteins* Molecular Sequence Data Nuclear Proteins* Organ Specificity Polymerase Chain Reaction RNA, Messenger / analysis Rats Rats, Sprague-Dawley Ribosomal Proteins / chemistry, genetics* Sequence Homology, Amino Acid Transcription, Genetic / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM32355/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Metalloproteins; 0/Nuclear Proteins; 0/RNA, Messenger; 0/Ribosomal Proteins; 0/Rps27 protein, rat |
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