| Evolution of a tissue-specific splicing network. | |
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MedLine Citation:
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PMID: 21406555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alternative splicing of precursor mRNA (pre-mRNA) is a strategy employed by most eukaryotes to increase transcript and proteomic diversity. Many metazoan splicing factors are members of multigene families, with each member having different functions. How these highly related proteins evolve unique properties has been unclear. Here we characterize the evolution and function of a new Drosophila splicing factor, termed LS2 (Large Subunit 2), that arose from a gene duplication event of dU2AF(50), the large subunit of the highly conserved heterodimeric general splicing factor U2AF (U2-associated factor). The quickly evolving LS2 gene has diverged from the splicing-promoting, ubiquitously expressed dU2AF(50) such that it binds a markedly different RNA sequence, acts as a splicing repressor, and is preferentially expressed in testes. Target transcripts of LS2 are also enriched for performing testes-related functions. We therefore propose a path for the evolution of a new splicing factor in Drosophila that regulates specific pre-mRNAs and contributes to transcript diversity in a tissue-specific manner. |
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Authors:
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J Matthew Taliaferro; Nehemiah Alvarez; Richard E Green; Marco Blanchette; Donald C Rio |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Genes & development Volume: 25 ISSN: 1549-5477 ISO Abbreviation: Genes Dev. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-16 Completed Date: 2011-05-05 Revised Date: 2012-04-26 |
Medline Journal Info:
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Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: United States |
Other Details:
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Languages: eng Pagination: 608-20 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Drosophila Proteins / genetics*, metabolism* Drosophila melanogaster / classification Evolution, Molecular* Gene Duplication / genetics Gene Expression Regulation Male Nuclear Proteins / genetics Organ Specificity Phylogeny Protein Splicing / genetics* Ribonucleoproteins / genetics Testis / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01GM61987/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Nuclear Proteins; 0/Ribonucleoproteins; 0/splicing factor U2AF |
| Comments/Corrections | |
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