| Evolution of oxygen and glucose concentration profiles in a tissue-mimetic culture system of embryonic stem cells. | |
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MedLine Citation:
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PMID: 16832606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A tissue-mimetic culture system (TMCS) in which cells are sandwiched between two glass slides provides an ideal microenvironment for studying the effects of oxygen and nutrient gradients on cells in culture. A mathematical model was utilized to predict the time course of the development of oxygen and glucose concentration gradients within the TMCS. Oxygen and glucose consumption rates of mouse embryonic stem cells were measured as parameters for the model. The model predicts oxygen and glucose concentration profiles directly using a single experimentally controlled variable, the seeding density of cells within the system. The model predicts that the time required for the gradients to reach steady state is inversely related to the cell density, and the penetration depth of the gradients into the TMCS is inversely related to the square root of the cell density. Experimental oxygen concentration measurements were performed at a cell density of 9.1 x 10(6) cells cm(-3), and the gradient was found to develop to a steady-state profile within 20 min and penetrate approximately 2 mm into the TMCS, consistent with the theoretical predictions. This model and the TMCS provide useful tools for investigating the effect of the metabolic microenvironment on cells in culture. |
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Authors:
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David M Cochran; Dai Fukumura; Marek Ancukiewicz; Peter Carmeliet; Rakesh K Jain |
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Publication Detail:
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Type: Journal Article Date: 2006-07-11 |
Journal Detail:
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Title: Annals of biomedical engineering Volume: 34 ISSN: 0090-6964 ISO Abbreviation: Ann Biomed Eng Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-09 Completed Date: 2006-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0361512 Medline TA: Ann Biomed Eng Country: United States |
Other Details:
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Languages: eng Pagination: 1247-58 Citation Subset: IM |
Affiliation:
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Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, 100 Blossom St, Cox-7, Boston, Massachusetts, 02114, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomimetics / methods* Cell Proliferation Cells, Cultured Computer Simulation Glucose / metabolism* Humans Kinetics Metabolic Clearance Rate Models, Biological* Oxygen / metabolism* Stem Cells / cytology*, physiology* Tissue Culture Techniques / methods* |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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